Custom Kit


PUREfrex® is a reconstituted cell-free protein synthesis system based on the PURE system, which is reconstituted from individually purified factors involved in protein synthesis in E.coli. It has an advantage that the composition of the reagent can be easily customized according to the purpose.

Custom kits are made to order based on PUREfrex® 1.0.

Components of PUREfrex® 1.0

Solution I (Buffer, Salts, Substrates)
Hepes-KOH pH 7.6
Potassium glutamate
Magnesium acetate
4 NTPs
20 Amino acids
Creatine phoshate
tRNA mixture
Solution II (Proteins)
20 Aminoacyl-tRNA synthetases (ARS)
Methionyl-tRNA transformylase (MTF)
3 Initiation factors (IF1, IF2, IF3)
3 Elongation factors (EF-Tu, EF-Ts, EF-G)
3 Release factors (RF1, RF2, RF3)
Ribosome recycling factor (RRF)
T7 RNA polymerase
Nucleoside diphosphate kinase (NDK)
Adenylate kinase (ADK, myokinase)
Ceatine kinase (CK)
Pyrophosphatase (PPiase)
Solution III


(-) RF1 For synthesis of a protein containing a noncanonical amino acid by amber suppression as below.
(-) T7 RNA polymerase For use of other RNA polymerase such as E.coli RNA polymerase.
(-) Specific amino acid(s) and/or
aminoacyl-tRNA synthetase
For synthesis of a protein containing noncanonical amino acid or less than 20 amino acids.

How to order

  1. Request an agreement and check the terms and conditions of use.
  2. Ask possibility if your designed kit could be prepared.
  3. Sign the agreement.
  4. Receive product number for your designed kit.
  5. Order with the received product number.


Custom kits are sold under the agreement of GeneFrontier (“GFC”). Rights to use this product are limited.

Condition of Use

  • Material is for internal research use only, and for non-clinical use;
  • Material is for non-commercial use, and not allowed to distribute to any third parties;
  • Material is not to be modified beyond the Purpose of Use;
  • Customer shall promptly notify GFC if any new invention is made through the use of Material or its portion;
  • Any new invention relating to Material and the use belongs to GFC;
  • Any products itself synthesized through the use of Material, and the ownership of the synthesized products belong to Customer.
  • Industrial customers need additional “License Agreement” for the use of in vitro display.

Technical Supports

For inquiries about the following contents, please contact GFC.

  • Request for the agreement
  • Whether the desired solution mixture can be prepared
  • Request for proposal of custom kit suitable for experiment purpose

Request for Quotation

Price is set for each reaction volume.
The minimum volume that can be ordered is 1 mL and the sales unit is 1 mL.
Please request a quote to Cosmo Bio USA.