We introduce the paper published by Takuya Ueda of the University of Tokyo who developed the PURE system which is the basic technology of PUREfrex^®.

In this paper, a reaction solution contains Release factors same as the commercially available PUREfrex^® kit. Therefore the stop codon is removed from the library.

J Biochem(2009)145(5):693-700 Osada
http://www.ncbi.nlm.nih.gov/pubmed/19228777

In addition, we recommend that you refer to the above mentioned paper especially the composition of buffers for ribosome display selection even when using a commercially available PUREfrex^® kit.

Sometimes the ribosome display may not work as expected since the commercially available PUREfrex^® kit is optimized for protein synthesis.

Please contact us for any questions or concerns.