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Influence of reducing reagents on the redox state
In PUREfrex®, a reducing reagent is added to mimic the cytos…… -
Improvement of specific activity by methylation of release factors (RF1 and RF2)
PUREfrex® is a cell-free protein synthesis system in which…… -
Improvement of the translation efficiency of consecutive proline residue-containing proteins by EF-P
When synthesizing proteins containing consecutive proline …… -
Synthesis of proteins containing disulfide bonds with PDI and DsbC
Sumarry We synthesized some model proteins, which have mult…… -
PUREfrex® RD: the unique antibody engineering method based on Ribosome Display with PUREfrex®
Recently, in vitro screening methods such as phage display o…… -
Synthesis of functionally active proteins having disulfide bonds
We synthesized vtPA and AppA as a model protein, which has m……
