Product instructions
-
HuCAL® -Technical Manual
-
PUREfrex® 2.0 (#PF201-0.25-5-EX) Instruction
-
PUREfrex® 2.0 (#PF201-0.25-EX) Instruction
-
PUREfrex® 2.1 (#PF213-0.25-5-EX) Instruction
-
PUREfrex® 2.1 (#PF213-0.25-EX) Instruction
-
DnaK Mix (#PF003-0.5-EX) Instruction
-
GroE Mix (#PF004-0.5-EX) Instruction
-
DsbC Set (#PF005-0.5-EX) Instruction (former DS supplement)
-
PDI Set (#PF006-0.5-EX) Instruction
Poster
-
In vitro selection from designed protein scaffold library with Ribosome Display on PURE system [2011]
Seventh Annual PEGS – The Essential Protein Engineering Summit
<Ribosome display, scaffold library> -
In vitro selection from designed protein scaffold library with Ribosome Display on PURE system [2011]
The 34th Annual Meeting of the Molecular Biology Society of Japan
<Ribosome display, scaffold library> -
In vitro affinity maturation based on Ribosome Display System with PUREfrex [2012]
8th Annual PEGS 2012 – the Essential Protein Engineering Summit
<Fab Maturation> -
In vitro affinity maturation based on Ribosome Display System with PUREfrex® [2012]
The 35th Annual Meeting of the Molecular Biology Society of Japan
<Fab antibody maturation> -
PUREfrex RD: the unique antibody engineering method based on Ribosome Display with PUREfrex® [2014]
10th Annual PEGS BOSTON – the essential protein engineering summit
<Fab, affinity maturation, EC50, KD> -
Simple and Effective Selection of cyclic peptides by Ribosome display with PUREfrex [2015]
PEGS Boston 2015 – the essential protein engineering summit
<bispecifics, epitope mapping/mimotope development> -
Simple and Effective Selection of cyclic peptides by Ribosome display with PUREfrex® [2015]
BMB2015 – Biochemistry and Molecular Biology in Kobe
<Fab+cyclic peptide, GFP+cyclic peptide (Binder A), epitope mapping/mimotope > -
Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
disulfide bounds
(Japanese language is partially included.) -
Performance of PUREfrex®2.0 with increased productivity and its application [2015]
new launched, 1 mg/mL
(Japanese language is partially included.) -
In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex®2.0 with increased productivity [2015]
Fab antibody, Immunotoxin
(Japanese language is partially included.) -
Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
Fab Heavy chain (Herceptin)
hPTGDS
Codon optimization of Hisx6-tag -
Efficient in vitro expression of aglycosylated full-length IgG using a reconstituted cell-free protein synthesis system, PUREfrex 2.0 [2016]
optimize the reaction condition for IgG synthesis
-
Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
membrane protein, various protein
(Japanese language is partially included.) -
Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex [2017]
Japanese language is partially included.
-
Preparation of labeled cyclic peptides with PUREfrex [2017]
ConBio2017 – Consortium of Biological Sciences in Kobe
<peptide labeled with biotin-Puromycin, CTL4 affinity maturation> -
Unique Cell-Free Protein Synthesis System, PUREfrex -Useful Platform for Protein Expression in the Development of Biologics- [2018]
In the poster session for PepTalk-The Protein Science Week: beginning January 08, 2018 in San Diego, CA.
-
Synthesis of proteins containing consecutive proline residues using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]
EF-P, consecutive proline
(Japanese language is partially included.) -
Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]
Proline stretch, Proline after the 1st Met, Proline before the stop codon, EF-P, RF2
(Japanese language partially included.) -
Selection and Affinity Maturation of Cyclic Peptide Against CTLA4 with PUREfrexRD [2019]
-
Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase [2018]
Optimization of the condition of reaction for synthesis proteins containing disulfide bonds.
hPDI, hEro1α, DsbC, GSSG
(Japanese language partially included.) -
In vitro selection and affinity maturation of CTLA-4 binding cyclic peptide with PUREfrexRD, and conversion of the peptide to small molecules. [2019]
The 42nd Annual Meeting of the Molecular Biology Society of Japan
December 3rd, 2019
Japanese is partially included. (PDF: 3MB) -
Synthesis of proteins containing disulfide bonds using a reconstituted cell-free protein synthesis system (PUREfrex). [2019]
Cell Free Systems Conference
December 4-6, 2019 Boston, MA U.S.A.
<PDF: 2MB> -
Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex) [2020]
The 43rd Annual Meeting of the Molecular Biology Society of Japan
December 2-4, 2020 Japan, Online.
[Optimization: DnaK conc. and timing of addition, Surfactant, Temperature. (hAChE, PPK2, Luciferase)]