YES.
PUREfrex^® is a reconstituted cell-free protein synthesis kit composed of E. coli ribosomes and translation factors, but eukaryotic proteins from such as mammalian and plant can also be synthesized.

The productivity of the target protein may depend on the property of nucleotide sequence encoding the target protein, such as GC contents or frequency of rare codon. The nucleotide sequence and amino acid sequence of the template DNA, rather than the origin of the protein, may affect the amount of the product, and optimizing the causative sequences may improve the amount of the protein synthesized.

For more information, please see Question 1 of “FAQ about the template DNA”.