YES.
PUREfrex^® uses a tRNA mixture prepared from E. coli, so each tRNA concentration reflects the tRNA concentration in E. coli. Therefore, the amount of tRNA that is low in E. coli is also low in the PUREfrex^® reaction solution.

For the above reasons, we recommend basically that the template DNA for PUREfrex^® should use codons that match the codon frequency in E. coli. In addition to that, when you design your template DNA, there are some more points to be considered, so please see Question 1 of “FAQ about the template DNA”.