Q13: Are the N-terminal methionines of proteins/peptides expressed in PUREfrex® formylated? If so, are they deformylated during/after translation?

YES.
The N-terminal methionine of the product synthesized by PUREfrex® is formylated. And they are not deformylated during/after translation.

However, depending on the amount of synthesized protein, the formyl donor may be depleted and some non-formylated proteins may be present. PUREfrex® proceeds with the translation reaction even if the N-terminal methionine is not formylated, but synthesis efficiency may be affected depending on the protein to be synthesized.

Related Products and Services

PUREfrex
PUREfrex®