Q4: Can multiple proteins be synthesized simultaneously?

Using multiple template DNAs encoding different proteins, it is possible to synthesize multiple proteins simultaneously in one tube. If the amount of synthesized protein differs from each template DNA, the amount of each products can be controlled by adjusting the ratio of template DNA to add.
As an example, you can see the results of simultaneous synthesis of immunoglobulin (IgG) light chain (LC) and heavy chain (HC) on the link here: “https://www.nature.com/articles/s41598-018-36691-8“.

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