Q8: Can I synthesize a membrane protein with PUREfrex®?

YES.
In most cases, a synthesized membrane protein may form aggregates. To obtain a membrane protein inserted into a lipid bilayer, you may add a lipid bilayer such as liposome or nanodisc into PUREfrex® when you synthesize the membrane protein.
Please see, “【Poster_MBSJ 2016】Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system”

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