YES.
Regarding magnesium ion, if the final concentration in the PUREfrex^® 1.0 reaction solution is only a few mM, there is little effect on protein synthesis. For other divalent cations, if the final concentration in the PUREfrex^® 1.0 reaction solution is 10 mM or more, the amount of product is often reduced, so avoid using them as much as possible.