Although unpurified PCR reaction mixture can be directly used with PUREfrex^®, do not add more than 10% (v/v) to the reaction mixture of PUREfrex^®. The efficiency of both transcription and translation reaction may decrease when the salt concentration is altered by carryover from the PCR mixture. If the concentration of the PCR product is low, prepare DNA solution with a sufficient concentration using a DNA purification kit instead of adding more unpurified PCR mixture to PUREfrex^®.