1) Add molecular chaperones when synthesizing the protein. PUREfrex^® contains no molecular chaperones. When the protein of interest is synthesized in the presence of molecular chaperones, it may become soluble. Additionally, better results may be obtained with PUREfrex^® 1.0, in which the yield of the product is lower than PUREfrex^® 2.0.

2) Synthesize the protein at a lower translation speed. Proteins generally fold more slowly than they are translated. Reducing the translation speed by lowering the reaction temperature from 37°C to 30°C or 25°C may increase the proportion of the target protein that is soluble. This will reduce the yield, so be sure to select conditions to efficiently produce the soluble protein.