Poster
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Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
disulfide bounds
(Japanese language is partially included.) -
Performance of PUREfrex®2.0 with increased productivity and its application [2015]
new launched, 1 mg/mL
(Japanese language is partially included.) -
In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex®2.0 with increased productivity [2015]
Fab antibody, Immunotoxin
(Japanese language is partially included.) -
Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
Fab Heavy chain (Herceptin)
hPTGDS
Codon optimization of Hisx6-tag -
Efficient in vitro expression of aglycosylated full-length IgG using a reconstituted cell-free protein synthesis system, PUREfrex 2.0 [2016]
optimize the reaction condition for IgG synthesis
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Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
membrane protein, various protein
(Japanese language is partially included.) -
Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex [2017]
Japanese language is partially included.
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Preparation of labeled cyclic peptides with PUREfrex [2017]
ConBio2017 – Consortium of Biological Sciences in Kobe
<peptide labeled with biotin-Puromycin, CTL4 affinity maturation> -
Unique Cell-Free Protein Synthesis System, PUREfrex -Useful Platform for Protein Expression in the Development of Biologics- [2018]
In the poster session for PepTalk-The Protein Science Week: beginning January 08, 2018 in San Diego, CA.
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Synthesis of proteins containing consecutive proline residues using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]
EF-P, consecutive proline
(Japanese language is partially included.) -
Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]
Proline stretch, Proline after the 1st Met, Proline before the stop codon, EF-P, RF2
(Japanese language partially included.) -
Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase [2018]
Optimization of the condition of reaction for synthesis proteins containing disulfide bonds.
hPDI, hEro1α, DsbC, GSSG
(Japanese language partially included.) -
Synthesis of proteins containing disulfide bonds using a reconstituted cell-free protein synthesis system (PUREfrex). [2019]
Cell Free Systems Conference
December 4-6, 2019 Boston, MA U.S.A.
<PDF: 2MB> -
Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex) [2020]
The 43rd Annual Meeting of the Molecular Biology Society of Japan
December 2-4, 2020 Japan, Online.
[Optimization: DnaK conc. and timing of addition, Surfactant, Temperature. (hAChE, PPK2, Luciferase)]