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Cell-free translation reconstituted with purified components.
Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, and Takuya Ueda -
A novel screening system for self-mRNA targeting proteins.
Bei-Wen Ying, Tsutomu Suzuki, Yoshihiro Shimizu, and Takuya Ueda -
Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.
Tsuyoshi Udagawa, Yoshihiro Shimizu, and Takuya Ueda -
Bulge- and basal layer-specific expression of fibroblast growth factor-13 (FHF-2) in mouse skin.
Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko Oki, and Toru Imamura -
Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import.
Takeyoshi Asai, Takashi Takahashi, Masatoshi Esaki, Shuh-Ichi Nishikawa, Kenzo Ohtsuka, Masato Nakai, and Toshiya Endo -
Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system.
Bei-Wen Ying, Hideki Taguchi, Hiroshi Ueda, and Takuya Ueda -
Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins.
Yutetsu Kuruma, Ken-Ichi Nishiyama, Yoshihiro Shimizu, Matthias Muller, and Takuya Ueda -
Novel processing and localization of catA; ccdA associated thiol-disulfide oxidoreductase; in protein hyper-producing bacterium Brevibacillus choshinensis.
Ryoichi Tanaka, Makoto Mizukami, and Masao Tokunaga -
Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides.
Bei-Wen Ying, Hideki Taguchi, Mayumi Kondo, and Takuya Ueda -
Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation.
Masato Yano, Hirotaka J Okano, and Hideyuki Okano -
Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.
Keiko Fukushima, Yukio Ikehara, and Katsuko Yamashita -
A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level.
Shinsuke Sando, Keiichiro Kanatani, Nobuhiko Sato, Hironori Matsumoto, Takahiro Hohsaka, and Yasuhiro Aoyama -
Protein synthesis by pure translation systems
Yoshihiro Shimizu, Takashi Kanamori, and Takuya Ueda -
Ribosomal synthesis of unnatural peptides.
Kristopher Josephson, Matthew C T Hartman, and Jack W Szostak -
Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library
Jarutat, T., Frisch, C., Nickels, C., Merz, H., Knappik, A. -
Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation.
Marc Hallier, Jessie Desreac, and Brice Felden -
Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains.
Sladjana Tomic, Arthur E Johnson, F Ulrich Hartl, and Stephanie A Etchells -
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Enhancement of protein synthesis by an inorganic polyphosphate in an E. coli cell-free system.
Hiromichi Itoh, Yumi Kawazoe, and Toshikazu Shiba -
Spermidine inhibits transient and stable ribosome subunit dissociation.
So Umekage, and Takuya Ueda -
A highly flexible tRNA acylation method for non-natural polypeptide synthesis.
Hiroshi Murakami, Atsushi Ohta, Hiroshi Ashigai, and Hiroaki Suga -
Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall.
Hiroki Muto, Hitoshi Nakatogawa, and Koreaki Ito -
Cytosolic chaperonin protects folding intermediates of Gbeta from aggregation by recognizing hydrophobic beta-strands. Proc. Natl.
Susumu Kubota, Hiroshi Kubota, and Kazuhiro Nagata -
Ribosomal synthesis of dehydroalanine-containing peptides.
Florian P Seebeck, and Jack W Szostak -
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Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli.
Tatsuya Yamamoto, Shunsuke Izumi, and Kunihiko Gekko -
Highly efficient ribosome display selection by use of purified components for in vitro translation.
Denis Villemagne, Ronald Jackson, and Julie A Douthwaite -
Affinity maturation of phage display antibody populations using ribosome display.
Maria Groves, Steven Lane, Julie Douthwaite, David Lowne, D Gareth Rees, Bryan Edwards, and Ronald H Jackson -
Regulation of mitochondrial morphology through proteolytic cleavage of OPA1.
Naotada Ishihara, Yuu Fujita, Toshihiko Oka, and Katsuyoshi Miharan -
Co-translational binding of GroEL to nascent polypeptides is followed by post-translational encapsulation by GroES to mediate protein folding.
Bei-Wen Ying, Hideki Taguchi, and Takuya Uedan -
Femtoliter compartment in liposomes for in vitro selection of proteins.
Takeshi Sunami, Kanetomo Sato, Tomoaki Matsuura, Koji Tsukada, Itaru Urabe, and Tetsuya Yomo -
Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.
Kiyoko Setoguchi, Hidenori Otera, and Katsuyoshi Mihara -
Selection of restriction endonucleases using artificial cells.
Yu Zheng, and Richard J Roberts -
Ribosomal protein S1 influences trans-translation in vitro and in vivo.
Matthieu Saguy, Reynald Gillet, Patricia Skorski, Sylvie Hermann-Le Denmat, and Brice Felden -
High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system.
Yuko Kawahashi, Nobuhide Doi, Yo Oishi, Chizuru Tsuda, Hideaki Takashima, Tomoya Baba, Hirotada Mori, Takashi Ito, and Hiroshi Yanagawa -
Structural and functional definition of the specificity of a novel caspase-3 inhibitor
Atsushi Yoshimori, Junichi Sakai, Satoshi Sunaga, Takanobu Kobayashi, Satoshi Takahashi, Naoyuki Okita, Ryoko Takasawa, and Sei-ichi Tanuma -
Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution.
Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando, and Yasuhiro Aoyama -
An mRNA-protein fusion at N-terminus for evolutionary protein engineering. Int.
Shingo Ueno, Hidenao Arai, Miho Suzuki, and Yuzuru Husimi -
Efficient protein selection based on ribosome display system with purified components.
Hiroyuki Ohashi, Yoshihiro Shimizu, Bei-Wen Ying, and Takuya Ueda -
Nascent chain; mRNA; and ribosome complexes generated by a pure translation system.
Tomoaki Matsuura, Hayato Yanagida, Junya Ushioda, Itaru Urabe, and Tetsuya Yomo -
Identification of nascent chain interaction sites on trigger factor.
Sathish K Lakshmipathy, Sladjana Tomic, Christian M Kaiser, Hung-Chun Chang, Pierre Genevaux, Costa Georgopoulos, Jose M Barral, Arthur E Johnson, F Ulrich Hartl, and Stephanie A Etchells -
Unexpected preference of the E. coli translation system for the ester bond during incorporation of backbone-elongated substrates.
Shinsuke Sando, Kenji Abe, Nobuhiko Sato, Toshihiro Shibata, Keigo Mizusawa, and Yasuhiro Aoyama -
Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II.
Kouji Kojima, Masaru Oshita, Yohei Nanjo, Koji Kasai, Yuzuru Tozawa, Hidenori Hayashi, and Yoshitaka Nishiyama -
A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites.
Cynthia M Sharma, Fabien Darfeuille, Titia H Plantinga, and Jorg Vogel -
Protein synthesis in liposomes with a minimal set of enzymes.
Giovanni Murtas, Yutetsu Kuruma, Paolo Bianchini, Alberto Diaspro, and Pier Luigi Luisi -
Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system.
Yoshio Doi, Takashi Ohtsuki, Yoshihiro Shimizu, Takuya Ueda, and Masahiko Sisido -
Synthesis of polyester by means of genetic code reprogramming.
Atsushi Ohta, Hiroshi Murakami, Eri Higashimura, and Hiroaki Suga -
Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue.
Hayato Yanagida, Tomoaki Matsuura, and Tetsuya Yomo -
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based.
Jason R Hillebrecht, and Shaorong Chong -
The BsaHI restriction-modification system: cloning; sequencing and analysis of conserved motifs.
Robert K Neely, and Richard J Roberts -
Messenger RNA-programmed incorporation of multiple N-methyl-amino acids into linear and cyclic peptides.
Takashi Kawakami, Hiroshi Murakami, and Hiroaki Suga -
Reprogramming the translation initiation for the synthesis of physiologically stable cyclic peptides.
Yuki Goto, Atsushi Ohta, Yusuke Sako, Yusuke Yamagishi, Hiroshi Murakami, and Hiroaki Suga -
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin.
Akihiko Sakamoto, Mai Yamagishi, Takafumi Watanabe, Youichi Aizawa, Takashi Kato, and Takashi Funatsu -
UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro.
Yoko Ozaki, Toshiharu Suzuki, Yutetsu Kuruma, Takuya Ueda, and Masasuke Yoshida -
Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
Johannes H Urban, and Jorg Vogel -
Ribosomal synthesis of peptidase-resistant peptides closed by a nonreducible inter-side-chain bond. ACS Chem. Biol. , 241-249.
Yusuke Sako, Yuki Goto, Hiroshi Murakami, and Hiroaki Suga -
Molecular mechanism of drug-dependent ribosome stalling.
Nora Vazquez-Laslop, Celine Thum, and Alexander S Mankin -
Ribosomal synthesis of N-methyl peptides.
Alexander O Subtelny, Matthew C T Hartman, and Jack W Szostak -
A general and rapid cell-free approach for the interrogation of protein-protein; protein-DNA; and protein-RNA interactions and their antagonists utilizing split-protein reporters.
Jason R Porter, Cliff I Stains, Benjamin W Jester, and Indraneel Ghosh -
Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions.
Yusuke Sako, Jumpei Morimoto, Hiroshi Murakami, and Hiroaki Suga -
Single-molecule imaging of full protein synthesis by immobilized ribosomes.
Sotaro Uemura, Ryo Iizuka, Taro Ueno, Yoshihiro Shimizu, Hideki Taguchi, Takuya Ueda, Joseph D Puglisi, and Takashi Funatsu -
Single-molecule imaging of full protein synthesis by immobilized ribosomes.
Sotaro Uemura, Ryo Iizuka, Taro Ueno, Yoshihiro Shimizu, Hideki Taguchi, Takuya Ueda, Joseph D Puglisi, and Takashi Funatsu -
RNA; but not protein partners; is directly responsible for translational silencing by a bacterial Hfq-binding small RNA.
Kimika Maki, Kanako Uno, Teppei Morita, and Hiroji Aiba -
Comprehensive analysis of the effects of Escherichia coli ORFs on protein translation reaction.
Yasuaki Kazuta, Jiro Adachi, Tomoaki Matsuura, Naoaki Ono, Hirotada Mori, and Tetsuya Yomo -
Cell-free synthesis of the torque-generating membrane proteins; PomA and PomB; of the Na+-driven flagellar motor in Vibrio alginolyticus.
Hiroyuki Terashima, Rei Abe-Yoshizumi, Seiji Kojima, and Michio Homma -
Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction.
Kazufumi Hosoda, Takeshi Sunami, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo -
Using shotgun sequence data to find active restriction enzyme genes.
Yu Zheng, Janos Posfai, Richard D Morgan, Tamas Vincze, and Richard J Roberts -
Quantifying epistatic interactions among the components constituting the protein translation system.
Tomoaki Matsuura, Yasuaki Kazuta, Takuyo Aita, Jiro Adachi, and Tetsuya Yomo -
Trigger factor from the psychrophilic bacterium Psychrobacter frigidicola is a monomeric chaperone.
Sylvain Robin, Denisio M Togashi, Alan G Ryder, and J Gerard Wall -
Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins.
Tatsuya Niwa, Bei-Wen Ying, Katsuyo Saito, WenZhen Jin, Shoji Takada, Takuya Ueda, and Hideki Taguchi -
Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.
Eriko Osada, Yoshihiro Shimizu, Bintang K Akbar, Takashi Kanamori, and Takuya Ueda -
Analysis of nonstop mRNA translation in the absence of tmRNA in Escherichia coli.
Kazushige Kuroha, Nobuko Horiguchi, Hiroji Aiba, and Toshifumi Inada -
Real-time monitoring of cell-free translation on a quartz-crystal microbalance.
Shuntaro Takahashi, Masaaki Iida, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda,and Yoshio Okahata -
Interleukin-33 is biologically active independently of caspase-1 cleavage.
Dominique Talabot-Ayer, Celine Lamacchia, Cem Gabay, and Gaby Palmer -
Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder.
Andre Estevez-Torres, Cecile Crozatier, Antoine Diguet, Tomoaki Hara, Hirohide Saito, Kenichi Yoshikawa, and Damien Baigl -
Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation.
Verena Pfeiffer, Kai Papenfort, Sacha Lucchini, Jay C D Hinton, and Jorg Vogel -
Identification of two active functional domains of human adenylate kinase 5.
Nicola Solaroli, Christakis Panayiotou, Magnus Johansson, and Anna Karlsson -
A new member of the Escherichia coli fad regulon: transcriptional regulation of fadM (ybaW).
Youjun Feng, and John E Cronan -
Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]
Ikuo Uchida, Ryoko Ishihara, Kiyoshi Tanaka, Eiji Hata, Sou-ichi Makino, Toru Kanno, Shinichi Hatama, Masato Kishima, Masato Akiba, Atsushi Watanabe, and Takayuki Kubota -
Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation.
Nora Gockler, Guillermo Jofre, Chrisovalantis Papadopoulos, Ulf Soppa, Francisco J Tejedor, and Walter Becker -
Roles of Tom70 in import of presequence-containing mitochondrial proteins.
Hayashi Yamamoto, Kenji Fukui, Hisashi Takahashi, Shingo Kitamura, Takuya Shiota, Kayoko Terao, Mayumi Uchida, Masatoshi Esaki, Shuh-ichi Nishikawa, Tohru Yoshihisa, Koji Yamano, and Toshiya Endo -
Profiling of gene-dependent translational progress in cell-free protein synthesis by real-space imaging.
Eriko Mikamo-Satoh, Akihiko Takagi, Hiroyuki Tanaka, Takuya Matsumoto, Tsutomu Nishihara, and Tomoji Kawai -
Bicistronic DNA display for in vitro selection of Fab fragments.
Takeshi Sumida, Nobuhide Doi, and Hiroshi Yanagawa -
Genetic identification of nascent peptides that induce ribosome stalling.
Douglas R Tanner, Daniel A Cariello, Christopher J Woolstenhulme, Mark A Broadbent, and Allen R Buskirk -
Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.
Osada E, Shimizu Y, Akbar BK, Kanamori T, Ueda T. -
mRNA display selection of a high-affinity; Bcl-XL-specific binding peptide.
Nobutaka Matsumura, Toru Tsuji, Takeshi Sumida, Masahito Kokubo, Michiko Onimaru, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato, and Hiroshi Yanagawa -
The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus.
Tomoko Noto, Henriette M Kurth, Kensuke Kataoka, Lucia Aronica, Leroi V DeSouza, K W Michael Siu, Ronald E Pearlman, Martin A Gorovsky, and Kazufumi Mochizuki -
A novel complete reconstitution system for membrane integration of the simplest membrane protein.
Ken-Ichi Nishiyama, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda, and Hajime Tokuda -
Comparing the functional properties of the Hsp70 chaperones; DnaK and BiP.
Jeanne Bonomo, John P Welsh, Karthish Manthiram, and James R Swartz -
Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions.
Takeshi Sunami, Kazufumi Hosoda, Hiroaki Suzuki, Tomoaki Matsuura, and Tetsuya Yomo -
Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis.
Erik Holmqvist, Johan Reimegard, Maaike Sterk, Nina Grantcharova, Ute Romling, and Eduard Gerhart Heinrich Wagner -
Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI.
Betty W Shen, Daniel F Heiter, Siu-Hong Chan, Hua Wang, Shuang-Yong Xu, Richard D Morgan, Geoffrey G Wilson, and Barry L Stoddard -
The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex.
Gandhi Theerthagiri, Nathalie Eisenhardt, Heinz Schwarz, and Wolfram Antonin -
In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme.
Haruichi Asahara, and Shaorong Chong -
Versatility of trigger factor interactions with ribosome-nascent chain complexes.
Sathish Kumar Lakshmipathy, Rashmi Gupta, Stefan Pinkert, Stephanie Anne Etchells, and F Ulrich Hartl -
Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation.
Yuki Moritani, Shin-ichiro M Nomura, Ikuo Morita, and Kazunari Akiyoshi -
PURE technology.
Yoshihiro Shimizu, and Takuya Ueda -
Production of multi-subunit complexes on liposome through an E. coli cell-free expression system.
Yutetsu Kuruma, Toshiharu Suzuki, and Takuya Ueda -
Ribosome display with the PURE technology.
Takuya Ueda, Takashi Kanamori, and Hiroyuki Ohashi -
Cortisol is involved in temperature-dependent sex determination in the Japanese flounder.
Toshiya Yamaguchi, Norifumi Yoshinaga, Takashi Yazawa, Koichiro Gen, and Takeshi Kitano -
In vitro selection of unnatural cyclic peptide libraries via mRNA display.
Zhong Ma, and Matthew C T Hartman -
Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae.
Marika Midon, Patrick Schafer, Alfred Pingoud, Mahua Ghosh, Andrea F Moon, Matthew J Cuneo, Robert E London, and Gregor Meiss -
Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries.
Tek N Lamichhane, N Dinuka Abeydeera, Anne-Cecile E Duc, Philip R Cunningham, and Christine S Chow -
Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP.
Vikram Narayan, Emmanuelle Pion, Vivien Landre, Petr Muller, and Kathryn L Ball -
The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes.
Christakis Panayiotou, Nicola Solaroli, Yunjian Xu, Magnus Johansson, and Anna Karlsson -
Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center.
Haripriya Ramu, Nora Vazquez-Laslop, Dorota Klepacki, Qing Dai, Joseph Piccirilli, Ronald Micura, and Alexander S Mankin -
YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes.
Yoshihiro Handa, Noriyuki Inaho, and Nobukazu Nameki -
Optimal codon choice can improve the efficiency and fidelity of N-methyl amino acid incorporation into peptides by in-vitro translation.
Shouji Yamamoto, Hidemasa Izumiya, Jiro Mitobe, Masatomo Morita, Eiji Arakawa, Makoto Ohnishi, and Haruo Watanabe -
Identification of a chitin-induced small RNA that regulates translation of the tfoX gene; encoding a positive regulator of natural competence in Vibrio cholerae.
Shouji Yamamoto, Hidemasa Izumiya, Jiro Mitobe, Masatomo Morita, Eiji Arakawa, Makoto Ohnishi, and Haruo Watanabe -
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, and Koreaki Ito -
Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome.
Zhan-Ping Zhou, Yoshihiro Shimizu, Hisashi Tadakuma, Hideki Taguchi, Koichi Ito, and Takuya Ueda -
Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox.
K Rosner, M F Kasprzak, A C J Horenstein, H L Thurston, J Abrams, L Y Kerwin, D A Mehregan, and D R Mehregan -
Improvement of translation efficiency in an Escherichia coli cell-free protein system using cysteine.
Tomoteru Shingaki, and Noriyuki Nimura -
tmRNA regulates synthesis of the ArfA ribosome rescue factor.
Fernando Garza-Sanchez, Ryan E Schaub, Brian D Janssen, and Christopher S Hayes -
Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants.
Federico De Masi, Christian A Grove, Anastasia Vedenko, Andreu Alibes, Stephen S Gisselbrecht, Luis Serrano, Martha L Bulyk, and Albertha J M Walhout -
Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays.
Kathy N Lam, Harm van Bakel, Atina G Cote, Anton van der Ven, and Timothy R Hughes -
Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes.
Takuma Ohtsuka, Satoshi Neki, Tamotsu Kanai, Kazunari Akiyoshi, Shin-Ichiro M Nomura, and Takashi Ohtsuki -
Kinetic study of de novo chromophore maturation of fluorescent proteins.
Ryo Iizuka, Mai Yamagishi-Shirasaki, and Takashi Funatsu -
Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5°C.
Fukashi Kihara, Teruyuki Niimi, Okitsugu Yamashita, and Toshinobu Yaginuma -
Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system.
John P Welsh, Jeanne Bonomo, and James R Swartz -
In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.
Hayato Yanagida, Tomoaki Matsuura, Yasuaki Kazuta, and Tetsuya Yomo -
Ribosome display selection of a murine IgG1 Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.
Sebastian Grimm, Feifan Yu, and Per-Ake Nygren -
Spontaneous crowding of ribosomes and proteins inside vesicles: a possible mechanism for the origin of cell metabolism. Chembiochem 12, 2325-2330.
Tereza Pereira de Souza, Frank Steiniger, Pasquale Stano, Alfred Fahr, and Pier Luigi Luisi -
Zn(II) binding to E. coli 70S ribosomes.
Mart Patrick Hensley, David L Tierney, and Michael Wade Crowder -
Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins.
Anna L Mallam, and Sophie E Jackson -
Luminescence Resonance Energy Transfer in the Cytoplasm of Live Escherichia coli Cells.
Daniel Gonzalez, Nayanish Lokhande, Swaraj Vadde, Qi Zhao, Aaron Cassill, and Robert Renthal -
Polypeptide aptamer selection using a stabilized ribosome display.
Wei Wang, Shuta Hara, Mingzhe Liu, Toshiro Aigaki, Shigeru Shimizu, and Yoshihiro Ito -
The ribosome modulates nascent protein folding.
Christian M Kaiser, Daniel H Goldman, John D Chodera, Ignacio Tinoco, and Carlos Bustamante -
Global analysis of chaperone effects using a reconstituted cell-free translation system.
Niwa T, Kanamori T, Ueda T, Taguchi H. -
Expression optimization and synthetic gene networks in cell-free systems.
David K Karig, Sukanya Iyer, Michael L Simpson, and Mitchel J Doktycz -
In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR.
Takeshi Sumida, Hiroshi Yanagawa, and Nobuhide Doi -
Directed evolution of proteins through in vitro protein synthesis in liposomes.
Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, and Tetsuya Yomo -
Peptide screening using PURE ribosome display.
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K Akbar, and Takuya Ueda -
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Characterization of type II and III restriction-modification systems from Bacillus cereus strains ATCC 10987 and ATCC 14579.
Shuang-Yong Xu, Rebecca L Nugent, Julie Kasamkattil, Alexey Fomenkov, Yogesh Gupta, Aneel Aggarwal, Xiaolong Wang, Zhiru Li, Yu Zheng, and Richard Morgan -
Synthesis of [11C]interleukin 8 using a cell-free translation system and L-[11C]methionine.
Ryuichi Harada, Shozo Furumoto, Takeo Yoshikawa, Yoichi Ishikawa, Katsuhiko Shibuya, Nobuyuki Okamura, Ren Iwata, and Kazuhiko Yanai -
DNA display selection of peptide ligands for a full-length human g protein-coupled receptor on CHO-K1 cells.
Nobuhide Doi, Natsuko Yamakawa, Hideaki Matsumoto, Yasutsugu Yamamoto, Tetsuya Nagano, Nobutaka Matsumura, Kenichi Horisawa, and Hiroshi Yanagawa -
Characterization of the emergent properties of a synthetic quasi-cellular system.
Lorenzo Lazzerini-Ospri, Pasquale Stano, Pier Luigi Luisi, and Roberto Marangoni -
A concept for selection of codon-suppressor tRNAs based on read-through ribosome display in an in vitro compartmentalized cell-free translation system.
Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando, and Yasuhiro Aoyama -
Clostridium difficile cell wall protein CwpV undergoes enzyme-independent intramolecular autoproteolysis.
Marcin Dembek, Catherine B Reynolds, and Neil F Fairweather -
Synchronized translation for detection of temporal stalling of ribosome during single-turnover translation.
Erik Holmqvist, Cecilia Unoson, Johan Reimegard, and E Gerhart H Wagner -
A mixed double negative feedback loop between the sRNA MicF and the global regulator Lrp.
Erik Holmqvist, Cecilia Unoson, Johan Reimegard, and E Gerhart H Wagner -
Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis.
Harris H Wang, Po-Yi Huang, George Xu, Wilhelm Haas, Adam Marblestone, Jun Li, Steven P Gygi, Anthony C Forster, Michael C Jewett, and George M Church -
Broad-specificity mRNA-rRNA complementarity in efficient protein translation.
Pamela A Barendt, Najaf A Shah, Gregory A Barendt, and Casim A Sarkar -
Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells.
Kodai Machida, Mamiko Masutani, Tominari Kobayashi, Satoshi Mikami, Yuri Nishino, Atsuo Miyazawa, and Hiroaki Imataka -
Real-time assay for testing components of protein synthesis.
Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Yale E Goldman, and Barry S Cooperman -
Triggered gene expression in fed-vesicle microreactors with a multifunctional membrane.
Zohreh Nourian, Wouter Roelofsen, and Christophe Danelon -
The ancestral SgrS RNA discriminates horizontally acquired Salmonella mRNAs through a single G-U wobble pair.
Kai Papenfort, Dimitri Podkaminski, Jay C D Hinton, and Jorg Vogel -
Traveling time of a translating ribosome along mRNA monitored directly on a quartz crystal microbalance.
Shuntaro Takahashi, Kentaro Tsuji, Takuya Ueda, and Yoshio Okahata -
In vitro selection of highly modified cyclic peptides that act as tight binding inhibitors. J. Am. Chem. Soc. 134 10469-10477.
Yollete V Guillen Schlippe, Matthew C T Hartman, Kristopher Josephson, and Jack W Szostak -
Differential regulation of mesodermal gene expression by Drosophila cell type-specific Forkhead transcription factors.
Xianmin Zhu, Shaad M Ahmad, Anton Aboukhalil, Brian W Busser, Yongsok Kim, Terese R Tansey, Adrian Haimovich, Neal Jeffries, Martha L Bulyk, and Alan M Michelson -
Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation.
Verena Kriechbaumer, Pengwei Wang, Chris Hawes, and Ben M Abell -
Mutant p53 cooperates with ETS2 to promote etoposide resistance.
Phi M Do, Lakshman Varanasi, Songqing Fan, Chunyang Li, Iwona Kubacka, Virginia Newman, Krishna Chauhan, Silvano Rakeem Daniels, Maurizio Boccetta, Michael R Garrett, Runzhao Li, and Luis A Martinez -
Experiment and mathematical modeling of gene expression dynamics in a cell-free system.
Tobias Stogbauer, Lukas Windhager, Ralf Zimmer, and Joachim O Radler -
A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.
Cassandra Guarino, and Matthew P Delisa -
Cell-free protein synthesis from a single copy of DNA in a glass microchamber.
Taiji Okano, Tomoaki Matsuura, Yasuaki Kazuta, Hiroaki Suzuki, and Tetsuya Yomo -
Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry.
Koji Nishimura, Tomoaki Matsuura, Kazuya Nishimura, Takeshi Sunami, Hiroaki Suzuki, and Tetsuya Yomo -
Target specificity of an autoreactive pathogenic human gd-T cell receptor in myositis.
Jessica Bruder, Katherina Siewert, Birgit Obermeier, Joachim Malotka, Peter Scheinert, Josef Kellermann, Takuya Ueda, Reinhard Hohlfeld, and Klaus Dornmair -
Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: Modeling of ligand-dependent and -independent expression.
Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, and Tetsuya Yomo -
Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.
Colin J B Harvey, Joseph D Puglisi, Vijay S Pande, David E Cane, and Chaitan Khosla -
Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli.
Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda, and Yoshitaka Nishiyama -
ArfA recruits RF2 into stalled ribosomes.
Yoshihiro Shimizu -
Analysis of p53 binding to DNA by fluorescence imaging microscopy.
Hwee Jing Ong, Jia Wei Siau, Jing Bo Zhang, Minghui Hong, Horst Flotow, and Farid Ghadessy -
Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis.
Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Norikazu Ichihashi, Hiroaki Suzuki, and Tetsuya Yomo -
Nanoproteomics enabling personalized nanomedicine.
Claudio Nicolini, Nicola Bragazzi, and Eugenia Pechkova -
Modification-free photocontrol of b-lactam conversion with spatiotemporal resolution.
Anna Venancio-Marques, Yan-Jun Liu, Antoine Diguet, Thomas di Maio, Arnaud Gautier, and Damien Baigl -
Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning.
Yasuhiro Fujino, Risako Fujita, Kouichi Wada, Kotomi Fujishige, Takashi Kanamori, Lindsey Hunt, Yoshihiro Shimizu, and Takuya Ueda -
SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice.
Yasuhiko Nakamura, Masahito Ogura, Kasane Ogura, Daisuke Tanaka, and Nobuya Inagaki -
Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming.
Ki Baek Lee, Ho-Cheol Kim, Dong-Myung Kim, Taek Jin Kang, and Hiroaki Suga -
Suppression of Gene Expression by G‐Quadruplexes in Open Reading Frames Depends on G‐Quadruplex Stability.
Endoh, T., Kawasaki, Y., & Sugimoto, N. -
Stability of RNA quadruplex in open reading frame determines proteolysis of human estrogen receptor α.
Endoh, T., Kawasaki, Y., & Sugimoto, N. -
In vitro evolution of α-hemolysin using a liposome display.
Fujii S, Matsuura T, Sunami T, Kazuta Y and Yomo T. -
Real-time mRNA measurement during an in vitro transcription and translation reaction using binary probes.
Henrike Niederholtmeyer, Ling Xu, and Sebastian J Maerkl -
In vitro integration of ribosomal RNA synthesis; ribosome assembly; and translation.
Michael C Jewett, Brian R Fritz, Laura E Timmerman, and George M Church -
Nascent peptides that block protein synthesis in bacteria.
Christopher J Woolstenhulme, Shankar Parajuli, David W Healey, Diana P Valverde, E Nicholas Petersen, Agata L Starosta, Nicholas R Guydosh, W Evan Johnson, Daniel N Wilson, and Allen R Buskirk -
Fluorescent proteins and in vitro genetic organization for cell-free synthetic biology.
Roberta Lentini, Michele Forlin, Laura Martini, Cristina Del Bianco, Amy C Spencer, Domenica Torino, and Sheref S Mansy -
Implementation of cell-free biological networks at steady state.
Henrike Niederholtmeyer, Viktoria Stepanova, and Sebastian J Maerkl -
Unbiased tracking of the progression of mRNA and protein synthesis in bulk and in liposome-confined reactions.
Pauline van Nies, Zohreh Nourian, Maurits Kok, Roeland van Wijk, Jonne Moeskops, Ilja Westerlaken, Jos M Poolman, Rienk Eelkema, Jan H van Esch, Yutetsu Kuruma, Takuya Ueda, and Christophe Danelon -
In vitro evolution of a-hemolysin using a liposome display.
Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta, and Tetsuya Yomo -
Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength.
Fabio Chizzolini, Michele Forlin, Dario Cecchi, and Sheref S Mansy -
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific non-standard amino acid incorporation.
Seok Hoon Hong, Ioanna Ntai, Adrian D Haimovich, Neil L Kelleher, Farren J Isaacs, and Michael C Jewett -
Translational halt during elongation caused by G-quadruplex formed by mRNA.
Tamaki Endoh, Yu Kawasaki, and Naoki Sugimoto -
Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system.
Kei Fujiwara, Tsutomu Katayama, and Shin-Ichiro M Nomura -
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PURE ribosome display and its application in antibody technology.
Kanamori T, Fujino Y and Ueda T. -
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Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.
Kazuta Y, Matsuura T, Ichihashi N and Yomo T. -
In vitro synthesis of the E. coli Sec translocon from DNA.
Matsubayashi, H., Kuruma, Y., & Ueda, T. -
Protein synthesis yield increased 72 times in the cell-free PURE system
Jackson, K., Kanamori, T., Ueda, T., & Fan, Z. H. -
The PURE System for Protein Production.
Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda -
Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.
Hiroyuki Kogure, Yoshihiro Handa, Masahiro Nagata, Naoto Kanai, Peter Guntert, Kenji Kubota, and Nobukazu Nameki -
Simple Identification of Two Causes of Noise in an Aptazyme System by Monitoring Cell-Free Transcription
Ichihashi, N., Kobori, S., & Yomo, T. -
Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells.
van Nies, P., Canton, A. S., Nourian, Z., & Danelon, C. -
In vitro directed evolution of alpha-hemolysin by liposome display.
Fujii S, Matsuura T and Yomo T. -
Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device.
Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H., & Takinoue, M. -
Droplet‐Shooting and Size‐Filtration (DSSF) Method for Synthesis of Cell‐Sized Liposomes with Controlled Lipid Compositions.
Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., ... & Takinoue, M. -
Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system in Escherichia coli.
Awai T, Ichihashi N and Yomo T. -
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Cotranslational protein folding inside the ribosome exit tunnel.
Nilsson, O. B., Hedman, R., Marino, J., Wickles, S., Bischoff, L., Johansson, M., ... & Beckmann, R. -
Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria.
Ishii, E., Chiba, S., Hashimoto, N., Kojima, S., Homma, M., Ito, K., ... & Mori, H. -
The thermotolerant yeast Kluyveromyces marxianus is a useful organism for structural and biochemical studies of autophagy.
Yamamoto, H., Shima, T., Yamaguchi, M., Mochizuki, Y., Hoshida, H., Kakuta, S., ... & Akada, R. -
Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system.
Niwa, T., Sasaki, Y., Uemura, E., Nakamura, S., Akiyama, M., Ando, M., ... & Akiyoshi, K. -
PURE mRNA display for in vitro selection of single-chain antibodies.
Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H., & Doi, N. -
Development of human neutralizing antibody to ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2).
Shiraishi, A., Mochizuki, S., Miyakoshi, A., Kojoh, K., & Okada, Y. -
Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method.
Ando, M., Akiyama, M., Okuno, D., Hirano, M., Ide, T., Sawada, S., ... & Akiyoshi, K. -
Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing.
Chadani, Y., Niwa, T., Chiba, S., Taguchi, H., & Ito, K. -
Trigger factor reduces the force exerted on the nascent chain by a cotranslationally folding protein.
Nilsson, O. B., Müller-Lucks, A., Kramer, G., Bukau, B., & von Heijne, G. -
GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription-Translation System.
Maddalena LL, Niederholtmeyer H, Turtola M, Swank ZN, Belogurov GA and Maerkl SJ. -
Feedback control of Campylobacter jejuni flagellin levels through reciprocal binding of FliW to flagellin and the global regulator CsrA.
Radomska, K. A., Ordoñez, S. R., Wösten, M. M., Wagenaar, J. A., & van Putten, J. P. -
In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display.
Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K., & Doi, N. -
In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display.
Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K., & Doi, N. -
Cell-free phospholipid biosynthesis by gene-encoded enzymes reconstituted in liposomes.
Scott, A., Noga, M. J., de Graaf, P., Westerlaken, I., Yildirim, E., & Danelon, C. -
Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27.
Fan, Y., Hoshino, T., & Nakamura, A. -
Cell-Free Translation Is More Variable than Transcription.
Chizzolini F, Forlin M, Yeh Martín N, Berloffa G, Cecchi D and Mansy SS. -
Cotranslational folding of spectrin domains via partially structured states.
Nilsson, O. B., Nickson, A. A., Hollins, J. J., Wickles, S., Steward, A., Beckmann, R., ... & Clarke, J. -
Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translation, whereas short L31 cleaved by protease 7 reduces both activities.
Ueta, M., Wada, C., Bessho, Y., Maeda, M., & Wada, A. -
Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system.
Li J, Zhang C, Huang P, Kuru E, Forster-Benson ETC, Li T and Church GM. -
Initiating translation with D-amino acids.
Yuki Goto, Hiroshi Murakami, and Hiroaki Suga -
Application of distributive conjugal DNA transfer in Mycobacterium smegmatis to establish a genome-wide synthetic genetic array.
Judd, J., Boucher, N., Van Roey, E., Gray, T. A., & Derbyshire, K. M. -
In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay).
Matsumoto, K. I., Yamazaki, K., Kawakami, S., Miyoshi, D., Ooi, T., Hashimoto, S., & Taguchi, S. -
De novo design and synthesis of a 30-cistron translation-factor module.
Shepherd, T. R., Du, L., Liljeruhm, J., Wang, J., Sjödin, M. O., Wetterhall, M., ... & Forster, A. C. -
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics.
Akaike, T., Ida, T., Wei, F. Y., Nishida, M., Kumagai, Y., Alam, M. M., ... & Nishimura, A. -
Intrinsic ribosome destabilization underlies translation and provides an organism with a strategy of environmental sensing.
Chadani, Y., Niwa, T., Izumi, T., Sugata, N., Nagao, A., Suzuki, T., ... & Taguchi, H. -
Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.
Katano, Y., Li, T., Baba, M., Nakamura, M., Ito, M., Kojima, K., ... & Yasukawa, K. -
Uncovering cell-free protein expression dynamics by a promoter library with diverse strengths.
Reyes, S. G., Kuruma, Y., & Tsuda, S. -
Ribosome incorporation into somatic cells promotes lineage transdifferentiation towards multipotency.
Ito, N., Katoh, K., Kushige, H., Saito, Y., Umemoto, T., Matsuzaki, Y., ... & Araki, N. -
Cotranslational protein assembly imposes evolutionary constraints on homomeric proteins.
Natan, E., Endoh, T., Haim-Vilmovsky, L., Flock, T., Chalancon, G., Hopper, J. T., ... & Pál, C. -
Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics.
Narumi R, Masuda K, Tomonaga T, Adachi J, Ueda HR and Shimizu Y. -
De novo synthesis of basal bacterial cell division proteins FtsZ, FtsA, and ZipA inside giant vesicles.
Furusato, T., Horie, F., Matsubayashi, H. T., Amikura, K., Kuruma, Y., & Ueda, T. -
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.
van Nies, P., Westerlaken, I., Blanken, D., Salas, M., Mencía, M., & Danelon, C. -
High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding.
Komura, R., Aoki, W., Motone, K., Satomura, A., & Ueda, M. -
Persulfide synthases function coupled with translation and mediate sulfur respiration.
Fuji, S., Sawa, T., Motohashi, H., & Akaike, T. -
Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ and Seeger MA. -
Development of Visible‐Light‐Responsive RNA Scissors Based on a 10–23 DNAzyme.
Kamiya, Y., Arimura, Y., Ooi, H., Kato, K., Liang, X. G., & Asanuma, H. -
Multitasking of Hsp70 chaperone in the biogenesis of bacterial functional amyloids.
Sugimoto, S., Arita-Morioka, K. I., Terao, A., Yamanaka, K., Ogura, T., & Mizunoe, Y. -
MifM-instructed translation arrest involves nascent chain interactions with the exterior as well as the interior of the ribosome.
Fujiwara, K., Ito, K., & Chiba, S. -
Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer.
Hayase, G., & Nomura, S. I. M. -
Liquid Marbles as an Easy‐to‐Handle Compartment for Cell‐Free Synthesis and In Situ Immobilization of Recombinant Proteins.
Kamiya, N., Ohama, Y., Minamihata, K., Wakabayashi, R., & Goto, M. -
G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System.
Gessesse, B., Nagaike, T., Nagata, K., Shimizu, Y., & Ueda, T. -
The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome.
Tian, P., Steward, A., Kudva, R., Su, T., Shilling, P. J., Nickson, A. A., ... & Clarke, J. -
Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome.
Marsden, A. P., Hollins, J. J., O’Neill, C., Ryzhov, P., Higson, S., Mendonça, C. A., ... & Clarke, J. -
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A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System.
Lavickova B and Maerkl SJ. -
Modelling cell-free RNA and protein synthesis with minimal systems.
Doerr, A., de Reus, E., van Nies, P., van der Haar, M., Wei, K., Kattan, J., ... & Danelon, C. -
Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system.
Murakami, S., Matsumoto, R., & Kanamori, T. -
Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System.
Moriizumi Y, Tabata KV, Miyoshi D and Noji H. -
Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip.
Misawa N, Fujii S, Kamiya K, Osaki T, Takaku T, Takahashi Y and Takeuchi S -
Artificial photosynthetic cell producing energy for protein synthesis.
Berhanu S, Ueda T and Kuruma Y -
Quantitative imaging of gene-expressing liposomes reveals rare favorable phenotypes.
Blanken D, van Nies P and Danelon C. -
Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch
Mohammed Dwidar, Yusuke Seike, Shungo Kobori, Charles Whitaker, Tomoaki Matsuura and Yohei Yokobayashi -
Programmable On-Chip Artificial Cell Producing Post-Translationally Modified Ubiquitinated Protein.
Zilberzwige-Tal S, Levin A, Toprakcioglu Z, Knowles TPJ, Gazit E and Elbaz J. -
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study
Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K and Hirata K. -
Genetically Encoded Membranes for Bottom‐Up Biology
Kilian Vogele, Tobias Pirzer and Friedrich C. Simmel -
Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
Yi Zhang, Yoshihiro Minagawa, Hiroto Kizoe, Kentaro Miyazaki, Ryota Iino, Hiroshi Ueno, Kazuhito V. Tabata, Yasuhiro Shimane and Hiroyuki Noji -
Centrifuge-based step emulsification device for simple and fast generation of monodisperse picoliter droplets
Dong-Chel Shina, Yuya Morimotoab, Jun Sawayamaa, Shigenori Miuraa and Shoji Takeuchi -
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.
Shimokawa-Chiba N, Müller C, Fujiwara K, Beckert B, Ito K, Wilson DN and Chiba S. -
Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens.
Su J, Kitaguchi T, Ohmuro-Matsuyama Y, Seah T, Ghadessy FJ, Hoon S and Ueda H. -
Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases.
Goto H, Kanai Y, Yotsui A, Shimokihara S, Shitara S, Oyobiki R, Fujiwara K, Watanabe T, Einaga Y, Matsumoto Y, Miki N and Doi N. -
Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest.
Muta M, Iizuka R, Niwa T, Guo Y, Taguchi H and Funatsu T. -
Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Hasebe T, Fujimoto K, Buchholz DR and Ishizuya-Oka A. -
The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.
Miyazaki R, Saiga H, Kato T, Bakoshi T, Senba R, Shintani A, Suzuki M, Takao K, Sasaki I, Iizuka A, Sugiyama M, Iwami N, Fukuda-Ohta Y, Hemmi H, Tanaka T, Miyake M, Kaisho T and Hoshino K. -
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.
Shimojo M, Amikura K, Masuda K, Kanamori T, Ueda T and Shimizu Y. -
Development of a monoclonal antibody for specific detection of Vibrio parahaemolyticus and analysis of its antigen.
Taro Yonekita, Naoki Morishita, Eiji Arakawa and Takashi Matsumoto. -
The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae.
Hayashi K, Ikeuchi T, Morishita R, Qian J, Kojima K, Takita T, Tanaka K, Hattori S and Yasukawa K. -
In Vitro Evolution of phi29 DNA Polymerases Through Compartmentalized Gene Expression and Rolling-Circle Replication
Yoshihiro Sakatani, Ryo Mizuuchi and Norikazu Ichihashi -
YkgM and YkgO Maintain Translation by Replacing Their Paralogs, Zinc-Binding Ribosomal Proteins L31 and L36, With Identical Activities
Masami Ueta, Chieko Wada and Akira Wada. -
Ribosome Rescue Activity of an Arabidopsis Thaliana ArfB Homolog
Michiaki Nagao, Fumina Tsuchiya, Reiko Motohashi and Tatsuhiko Abo. -
Reconstituted Cell-Free Protein Synthesis Using in Vitro Transcribed tRNAs
Keita Hibi, Kazuaki Amikura, Naoki Sugiura, Keiko Masuda, Satoshi Ohno, Takashi Yokogawa, Takuya Ueda and Yoshihiro Shimizu. -
Enzymatic Regulation and Biological Functions of Reactive Cysteine Persulfides and Polysulfides
Tomohiro Sawa , Hozumi Motohashi , Hideshi Ihara and Takaaki Akaike -
CoLiDe: Combinatorial Library Design tool for probing protein sequence space
Vyacheslav Tretyachenko, Václav Voráček, Radko Souček, Kosuke Fujishima and Klára Hlouchová. -
Proteome-wide Capture of Co-translational Protein Dynamics in Bacillus subtilis Using TnDR, a Transposable Protein-Dynamics Reporter
Keigo Fujiwara, Yutaro Katagi, Koreaki Ito and Shinobu Chiba. -
Reconstitution of mammalian mitochondrial translation system capable of correct initiation and long polypeptide synthesis from leaderless mRNA
Muhoon Lee, Noriko Matsunaga, Shiori Akabane, Ippei Yasuda, Takuya Ueda and Nono Takeuchi-Tomita. -
Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers
Tetsuya Yamada, Hirotaka Sugiura, Hisatoshi Mimura, Koki Kamiya, Toshihisa Osaki and Shoji Takeuchi. -
PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high-throughput sequencing
Sabrina Galiñanes Reyes, Yutetsu Kuruma, Mai Fujimi, Masako Yamazaki, Sumie Eto, Shota Nishikawa, Satoshi Tamaki, Asaki Kobayashi, Ryo Mizuuchi, Lynn Rothschild, Mark Ditzler and Kosuke Fujishima. -
Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways
Karen Sakiyama, Naomi Shimokawa-Chiba, Keigo Fujiwara and Shinobu Chiba. -
Cell-Free Expression of a Plant Membrane Protein BrPT2 From Boesenbergia Rotunda
Yvonne Jing Mei Liew, Yean Kee Lee, Norzulaani Khalid, Noorsaadah Abd Rahman and Boon Chin Tan. -
Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface
Michael Levy, Reuven Falkovich, Ohad Vonshak, Dan Bracha, Alexandra M Tayar, Yoshihiro Shimizu, Shirley S Daubev and Roy H Bar-Ziv.
