Poster

  • Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]

    disulfide bounds
    (Japanese language is partially included.)

  • Performance of PUREfrex®2.0 with increased productivity and its application [2015]

    new launched, 1 mg/mL
    (Japanese language is partially included.)

  • In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex®2.0 with increased productivity [2015]

    Fab antibody, Immunotoxin
    (Japanese language is partially included.)

  • Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]

    Fab Heavy chain (Herceptin)
    hPTGDS
    Codon optimization of Hisx6-tag

  • Efficient in vitro expression of aglycosylated full-length IgG using a reconstituted cell-free protein synthesis system, PUREfrex 2.0 [2016]

    optimize the reaction condition for IgG synthesis

  • Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]

    membrane protein, various protein
    (Japanese language is partially included.)

  • Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex [2017]

    Japanese language is partially included.

  • Preparation of labeled cyclic peptides with PUREfrex [2017]

    ConBio2017 – Consortium of Biological Sciences in Kobe
    <peptide labeled with biotin-Puromycin, CTL4 affinity maturation>

  • Unique Cell-Free Protein Synthesis System, PUREfrex -Useful Platform for Protein Expression in the Development of Biologics- [2018]

    In the poster session for PepTalk-The Protein Science Week: beginning January 08, 2018 in San Diego, CA.

  • Synthesis of proteins containing consecutive proline residues using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]

    EF-P, consecutive proline
    (Japanese language is partially included.)

  • Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis system (PUREfrex) [2018]

    Proline stretch, Proline after the 1st Met, Proline before the stop codon, EF-P, RF2
    (Japanese language partially included.)

  • Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase [2018]

    Optimization of the condition of reaction for synthesis proteins containing disulfide bonds.
    hPDI, hEro1α, DsbC, GSSG
    (Japanese language partially included.)

  • Synthesis of proteins containing disulfide bonds using a reconstituted cell-free protein synthesis system (PUREfrex). [2019]

    Cell Free Systems Conference
    December 4-6, 2019  Boston, MA U.S.A.
    <PDF: 2MB>

  • Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex) [2020]

    The 43rd Annual Meeting of the Molecular Biology Society of Japan
    December 2-4, 2020 Japan, Online.
    [Optimization: DnaK conc. and timing of addition, Surfactant, Temperature. (hAChE, PPK2, Luciferase)]