Q7: Can I synthesize proteins containing disulfide bonds (such as antibodies) with PUREfrex®?

YES.
DsbC Set (#PF005-0.5-EX) or PDI Set (#PF006-05-EX) are supplements for PUREfrex® to add for synthesizing proteins containing disulfide bonds in active form. PUREfrex® 2.1 (#PF213-0.25-EX) is recommended to optimize the reaction conditions.
For antibodies (IgG, Fab, scFv) synthesis, please see the link: “https://www.nature.com/articles/s41598-018-36691-8“.

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