YES.
It is not possible with PUREfrex^® alone, as it does not contain post-translational modification enzymes.
However, synthesis is possible by adding post-translational modification enzymes and substrates to the reaction mixture.
For glycosylation, please see “【Poster_MBSJ 2021】 Synthesis of glycosylated proteins using PUREfrex^® with glycosyltransferase”.
For N-terminal modifications, please see the link: “https://pubs.acs.org/doi/10.1021/acssynbio.3c00191“.