PUREfrex® kit is a newly developed reconstituted cell-free protein synthesis reagent based on PURE system technology invented by Professor Takuya Ueda in the University of Tokyo. This reaction system consists of proteins, ribosome, amino acids and NTPs only (Ref. 1, 2). Those proteins are necessary for transcription, translation and energy regeneration. The proteins and ribosome are highly purified individually and assembled together to constitute the protein synthesis system. To synthesize your protein, just add your template DNA or mRNA encoding the protein of interest into reaction mixture, and incubate for several hours. This system’s biggest point is RECONSTITUTED system by assembling translation related factors only. By this unique character, you may adjust the composition of reaction mixture as you like and may not have to consider serious background for your downstream application.
HuCAL® stands for Human Combinatorial Antibody Library, a unique synthetic collection containing billions of recombinant human Fab antibodies which encompass the human antibody repertoire.
HuCAL® technology represents the cutting edge of monoclonal antibody generation:
Ribosome display utilizing customized, PUREfrex®. Effective in vitro selection method for highly diversified protein libraries (>10bil) beyond phage display.
For screening of scFv, scaffold, etc.
We have been collaborating with MorphoSys AG, and introducing their proprietary technologies to Japanese market. Please see their web site for further information on their technologies.