*For the customers abroad, min quantity, shipping cost, insurance are to be discussed in each order.
PUREfrex® kit is a reagent pursued of the original concept of PURE system technology. The PURE system is a reconstituted cell-free protein synthesis system which consists of only purified factors. Those factors are ribome and proteins which is necessary for transcription, translation and energy regeneration.
PUREfrex® kit contains DTT. So your protein is synthesized under reducing environment. If your protein requires formation of disulfide bond, please see PUREfrex®SS (#PF002).

*1) Indicated temperature shows storage temperature after opening the kit. Please store the kit at -80°C before opening.
*2) The rest of solution should be frozen rapidly in liquid nitrogen or dry ice/ethanol, and be stored at -80°C. Divide into aliquots, if necessary, and avoid refreeze and thaw as much as possible.
*3) For synthesizing DHFR, add 2.5 µL of DHFR DNA to 50 µL of reaction.

Protocol

The amounts given are for a 50 µL reaction. For scaling up the reaction, adjust the volume of reagents accordingly.
Thaw Solution I by incubation at 30°C for 1 minute, and then cool on ice.
Thaw Solution II and III on ice.
Mix Solution I, II and III respectively by vortex and centrifuge briefly to collect each solution at the bottom.
Assemble the reaction mixture in a tube as follows. Add the template DNA to 1 – 3 ng/µL per 1 kb.

Incubate the tube at 37°C for 2 – 4 hours.
Analyze the synthesized products.

Note

PUREfrex® is developed for in vitro research only. PUREfrex® should not be used for the therapy, diagnostic or administration to animals including human and should not be used as food or cosmetics etc. To avoid the contamination of nuclease, nuclease-free-treated water, reagents and materials should be used. We also recommend wearing gloves and mask.
“PUREfrex® is Registered in U.S. Patent and Trademark Office”