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  • Influence of reducing reagents on the redox state

    In PUREfrex®, a reducing reagent is added to mimic the cytos……
    PUREfrex®
    2021
  • Improvement of specific activity by methylation of release factors (RF1 and RF2)

    PUREfrex® is a cell-free protein synthesis system in which……
    PUREfrex®
    2021
  • Improvement of the translation efficiency of consecutive proline residue-containing proteins by EF-P

    When synthesizing proteins containing consecutive proline ……
    PUREfrex®
    2020
  • Synthesis of proteins containing disulfide bonds with PDI and DsbC

    Sumarry We synthesized some model proteins, which have mult……
    PUREfrex®
    2019
  • Synthesis of functionally active proteins having disulfide bonds

    We synthesized vtPA and AppA as a model protein, which has m……
    PUREfrex®
    2012
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