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Rational Strategy for Designing Peptidomimetic Small Molecules Based on Cyclic Peptides Targeting Protein–Protein Interaction between CTLA-4 and B7-1
Kumiko Tsuihiji, Eiji Honda, Kanehisa Kojoh, Shizue Katoh, Tomonori Taguri, Atsushi Yoshimori, Hajime Takashima -
Chemical Synthesis and Cell-Free Expression of Thiazoline Ring-Bridged Cyclic Peptides and Their Properties on Biomembrane Permeability
Takashi Tamura, Masaaki Inoue, Yuji Yoshimitsu, Ichihiko Hashimoto, Noriyuki Ohashi, Kyosuke Tsumura, Koo Suzuki, Takayoshi Watanabe and Takahiro Hohsaka. -
Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display
Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe and Tomoaki Matsuura. -
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Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications
Kazuaki Amikura, Keita Hibi and Yoshihiro Shimizu. -
Embedding a membrane protein into an enveloped artificial viral replica
Hiroto Furukawa, Hiroshi Inaba, Yoshihiro Sasaki, Kazunari Akiyoshic and Kazunori Matsuura. -
Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system
Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori and Tomoaki Matsuura. -
In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins
Ryo Aoyama, Keiko Masuda, Masaru Shimojo, Takashi Kanamori, Takuya Ueda and Yoshihiro Shimizu. -
Structure-activity relationship for the folding intermediate-selective inhibition of DYRK1A
Yuka Miyazaki, Masaki Kikuchi, Koji Umezawa, Aurelie Descamps, Daichi Nakamura, Gaku Furuie, Tomoe Sumida, Kanako Saito, Ninako Kimura, Takashi Niwa, Yuto Sumid, Takashi Umehara, Takamitsu Hosoya and Isao Kii. -
Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation
Yuhei Chadani, Nobuyuki Sugata, Tatsuya Niwa, Yosuke Ito, Shintaro Iwasaki and Hideki Taguchi. -
Insect odorant receptor-based biosensors: Current status and prospects
Jamal Ahmed Cheema, Colm Carraher, Natalie O V Plank, Jadranka Travas-Sejdic and Andrew Kralicek. -
Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules
Johannes Kattan, Anne Doerr, Marileen Dogterom and Christophe Danelon. -
Discovery of a small protein-encoding cis-regulatory overlapping gene of the tumor suppressor gene Scribble in humans
Yuhta Nomura and Naoshi Dohmae. -
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A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection
Tingting Li, Hongmin Cai, ... and Dianfan Li. -
Cell free protein synthesis versus yeast expression – a comparison using insulin as a model protein
Astrid B Jensen, Franta Hubálek, Carsten Enggaard Stidsen, Eva Johansson, Fredrik Kryh Öberg, Michael Skjøt and Thomas Kjeldsen. -
Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface
Michael Levy, Reuven Falkovich, Ohad Vonshak, Dan Bracha, Alexandra M Tayar, Yoshihiro Shimizu, Shirley S Daubev and Roy H Bar-Ziv. -
Cell-Free Expression of a Plant Membrane Protein BrPT2 From Boesenbergia Rotunda
Yvonne Jing Mei Liew, Yean Kee Lee, Norzulaani Khalid, Noorsaadah Abd Rahman and Boon Chin Tan. -
Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways
Karen Sakiyama, Naomi Shimokawa-Chiba, Keigo Fujiwara and Shinobu Chiba. -
PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high-throughput sequencing
Sabrina Galiñanes Reyes, Yutetsu Kuruma, Mai Fujimi, Masako Yamazaki, Sumie Eto, Shota Nishikawa, Satoshi Tamaki, Asaki Kobayashi, Ryo Mizuuchi, Lynn Rothschild, Mark Ditzler and Kosuke Fujishima. -
Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers
Tetsuya Yamada, Hirotaka Sugiura, Hisatoshi Mimura, Koki Kamiya, Toshihisa Osaki and Shoji Takeuchi. -
Reconstitution of mammalian mitochondrial translation system capable of correct initiation and long polypeptide synthesis from leaderless mRNA
Muhoon Lee, Noriko Matsunaga, Shiori Akabane, Ippei Yasuda, Takuya Ueda and Nono Takeuchi-Tomita. -
Proteome-wide Capture of Co-translational Protein Dynamics in Bacillus subtilis Using TnDR, a Transposable Protein-Dynamics Reporter
Keigo Fujiwara, Yutaro Katagi, Koreaki Ito and Shinobu Chiba. -
CoLiDe: Combinatorial Library Design tool for probing protein sequence space
Vyacheslav Tretyachenko, Václav Voráček, Radko Souček, Kosuke Fujishima and Klára Hlouchová. -
Enzymatic Regulation and Biological Functions of Reactive Cysteine Persulfides and Polysulfides
Tomohiro Sawa , Hozumi Motohashi , Hideshi Ihara and Takaaki Akaike -
Reconstituted Cell-Free Protein Synthesis Using in Vitro Transcribed tRNAs
Keita Hibi, Kazuaki Amikura, Naoki Sugiura, Keiko Masuda, Satoshi Ohno, Takashi Yokogawa, Takuya Ueda and Yoshihiro Shimizu. -
Ribosome Rescue Activity of an Arabidopsis Thaliana ArfB Homolog
Michiaki Nagao, Fumina Tsuchiya, Reiko Motohashi and Tatsuhiko Abo. -
YkgM and YkgO Maintain Translation by Replacing Their Paralogs, Zinc-Binding Ribosomal Proteins L31 and L36, With Identical Activities
Masami Ueta, Chieko Wada and Akira Wada. -
In Vitro Evolution of phi29 DNA Polymerases Through Compartmentalized Gene Expression and Rolling-Circle Replication
Yoshihiro Sakatani, Ryo Mizuuchi and Norikazu Ichihashi -
The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae.
Hayashi K, Ikeuchi T, Morishita R, Qian J, Kojima K, Takita T, Tanaka K, Hattori S and Yasukawa K. -
Development of a monoclonal antibody for specific detection of Vibrio parahaemolyticus and analysis of its antigen.
Taro Yonekita, Naoki Morishita, Eiji Arakawa and Takashi Matsumoto. -
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.
Shimojo M, Amikura K, Masuda K, Kanamori T, Ueda T and Shimizu Y. -
The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.
Miyazaki R, Saiga H, Kato T, Bakoshi T, Senba R, Shintani A, Suzuki M, Takao K, Sasaki I, Iizuka A, Sugiyama M, Iwami N, Fukuda-Ohta Y, Hemmi H, Tanaka T, Miyake M, Kaisho T and Hoshino K. -
Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Hasebe T, Fujimoto K, Buchholz DR and Ishizuya-Oka A. -
Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest.
Muta M, Iizuka R, Niwa T, Guo Y, Taguchi H and Funatsu T. -
Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases.
Goto H, Kanai Y, Yotsui A, Shimokihara S, Shitara S, Oyobiki R, Fujiwara K, Watanabe T, Einaga Y, Matsumoto Y, Miki N and Doi N. -
Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens.
Su J, Kitaguchi T, Ohmuro-Matsuyama Y, Seah T, Ghadessy FJ, Hoon S and Ueda H. -
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.
Shimokawa-Chiba N, Müller C, Fujiwara K, Beckert B, Ito K, Wilson DN and Chiba S. -
Centrifuge-based step emulsification device for simple and fast generation of monodisperse picoliter droplets
Dong-Chel Shina, Yuya Morimotoab, Jun Sawayamaa, Shigenori Miuraa and Shoji Takeuchi -
Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
Yi Zhang, Yoshihiro Minagawa, Hiroto Kizoe, Kentaro Miyazaki, Ryota Iino, Hiroshi Ueno, Kazuhito V. Tabata, Yasuhiro Shimane and Hiroyuki Noji -
Genetically Encoded Membranes for Bottom‐Up Biology
Kilian Vogele, Tobias Pirzer and Friedrich C. Simmel -
Programmable On-Chip Artificial Cell Producing Post-Translationally Modified Ubiquitinated Protein.
Zilberzwige-Tal S, Levin A, Toprakcioglu Z, Knowles TPJ, Gazit E and Elbaz J. -
Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch
Mohammed Dwidar, Yusuke Seike, Shungo Kobori, Charles Whitaker, Tomoaki Matsuura and Yohei Yokobayashi -
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study
Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K and Hirata K. -
Quantitative imaging of gene-expressing liposomes reveals rare favorable phenotypes.
Blanken D, van Nies P and Danelon C. -
Artificial photosynthetic cell producing energy for protein synthesis.
Berhanu S, Ueda T and Kuruma Y -
Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip.
Misawa N, Fujii S, Kamiya K, Osaki T, Takaku T, Takahashi Y and Takeuchi S -
Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System.
Moriizumi Y, Tabata KV, Miyoshi D and Noji H. -
Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system.
Murakami, S., Matsumoto, R., & Kanamori, T. -
Modelling cell-free RNA and protein synthesis with minimal systems.
Doerr, A., de Reus, E., van Nies, P., van der Haar, M., Wei, K., Kattan, J., ... & Danelon, C. -
A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System.
Lavickova B and Maerkl SJ. -
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Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome.
Marsden, A. P., Hollins, J. J., O’Neill, C., Ryzhov, P., Higson, S., Mendonça, C. A., ... & Clarke, J. -
The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome.
Tian, P., Steward, A., Kudva, R., Su, T., Shilling, P. J., Nickson, A. A., ... & Clarke, J. -
G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System.
Gessesse, B., Nagaike, T., Nagata, K., Shimizu, Y., & Ueda, T. -
Liquid Marbles as an Easy‐to‐Handle Compartment for Cell‐Free Synthesis and In Situ Immobilization of Recombinant Proteins.
Kamiya, N., Ohama, Y., Minamihata, K., Wakabayashi, R., & Goto, M. -
Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer.
Hayase, G., & Nomura, S. I. M. -
MifM-instructed translation arrest involves nascent chain interactions with the exterior as well as the interior of the ribosome.
Fujiwara, K., Ito, K., & Chiba, S. -
Multitasking of Hsp70 chaperone in the biogenesis of bacterial functional amyloids.
Sugimoto, S., Arita-Morioka, K. I., Terao, A., Yamanaka, K., Ogura, T., & Mizunoe, Y. -
Development of Visible‐Light‐Responsive RNA Scissors Based on a 10–23 DNAzyme.
Kamiya, Y., Arimura, Y., Ooi, H., Kato, K., Liang, X. G., & Asanuma, H. -
Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ and Seeger MA. -
Persulfide synthases function coupled with translation and mediate sulfur respiration.
Fuji, S., Sawa, T., Motohashi, H., & Akaike, T. -
High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding.
Komura, R., Aoki, W., Motone, K., Satomura, A., & Ueda, M. -
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.
van Nies, P., Westerlaken, I., Blanken, D., Salas, M., Mencía, M., & Danelon, C. -
De novo synthesis of basal bacterial cell division proteins FtsZ, FtsA, and ZipA inside giant vesicles.
Furusato, T., Horie, F., Matsubayashi, H. T., Amikura, K., Kuruma, Y., & Ueda, T. -
Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics.
Narumi R, Masuda K, Tomonaga T, Adachi J, Ueda HR and Shimizu Y. -
Cotranslational protein assembly imposes evolutionary constraints on homomeric proteins.
Natan, E., Endoh, T., Haim-Vilmovsky, L., Flock, T., Chalancon, G., Hopper, J. T., ... & Pál, C. -
Ribosome incorporation into somatic cells promotes lineage transdifferentiation towards multipotency.
Ito, N., Katoh, K., Kushige, H., Saito, Y., Umemoto, T., Matsuzaki, Y., ... & Araki, N. -
Uncovering cell-free protein expression dynamics by a promoter library with diverse strengths.
Reyes, S. G., Kuruma, Y., & Tsuda, S. -
Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.
Katano, Y., Li, T., Baba, M., Nakamura, M., Ito, M., Kojima, K., ... & Yasukawa, K. -
Intrinsic ribosome destabilization underlies translation and provides an organism with a strategy of environmental sensing.
Chadani, Y., Niwa, T., Izumi, T., Sugata, N., Nagao, A., Suzuki, T., ... & Taguchi, H. -
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics.
Akaike, T., Ida, T., Wei, F. Y., Nishida, M., Kumagai, Y., Alam, M. M., ... & Nishimura, A. -
De novo design and synthesis of a 30-cistron translation-factor module.
Shepherd, T. R., Du, L., Liljeruhm, J., Wang, J., Sjödin, M. O., Wetterhall, M., ... & Forster, A. C. -
In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay).
Matsumoto, K. I., Yamazaki, K., Kawakami, S., Miyoshi, D., Ooi, T., Hashimoto, S., & Taguchi, S. -
Application of distributive conjugal DNA transfer in Mycobacterium smegmatis to establish a genome-wide synthetic genetic array.
Judd, J., Boucher, N., Van Roey, E., Gray, T. A., & Derbyshire, K. M. -
Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system.
Li J, Zhang C, Huang P, Kuru E, Forster-Benson ETC, Li T and Church GM. -
Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translation, whereas short L31 cleaved by protease 7 reduces both activities.
Ueta, M., Wada, C., Bessho, Y., Maeda, M., & Wada, A. -
Cotranslational folding of spectrin domains via partially structured states.
Nilsson, O. B., Nickson, A. A., Hollins, J. J., Wickles, S., Steward, A., Beckmann, R., ... & Clarke, J. -
Cell-Free Translation Is More Variable than Transcription.
Chizzolini F, Forlin M, Yeh Martín N, Berloffa G, Cecchi D and Mansy SS. -
Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27.
Fan, Y., Hoshino, T., & Nakamura, A. -
Cell-free phospholipid biosynthesis by gene-encoded enzymes reconstituted in liposomes.
Scott, A., Noga, M. J., de Graaf, P., Westerlaken, I., Yildirim, E., & Danelon, C. -
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In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display.
Nakayama M, Komiya S, Fujiwara K, Horisawa K and Doi N. -
In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display.
Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K., & Doi, N. -
Feedback control of Campylobacter jejuni flagellin levels through reciprocal binding of FliW to flagellin and the global regulator CsrA.
Radomska, K. A., Ordoñez, S. R., Wösten, M. M., Wagenaar, J. A., & van Putten, J. P. -
GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription-Translation System.
Maddalena LL, Niederholtmeyer H, Turtola M, Swank ZN, Belogurov GA and Maerkl SJ. -
Trigger factor reduces the force exerted on the nascent chain by a cotranslationally folding protein.
Nilsson, O. B., Müller-Lucks, A., Kramer, G., Bukau, B., & von Heijne, G. -
Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing.
Chadani, Y., Niwa, T., Chiba, S., Taguchi, H., & Ito, K. -
Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method.
Ando, M., Akiyama, M., Okuno, D., Hirano, M., Ide, T., Sawada, S., ... & Akiyoshi, K. -
Development of human neutralizing antibody to ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2).
Shiraishi, A., Mochizuki, S., Miyakoshi, A., Kojoh, K., & Okada, Y. -
PURE mRNA display for in vitro selection of single-chain antibodies.
Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H., & Doi, N. -
Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system.
Niwa, T., Sasaki, Y., Uemura, E., Nakamura, S., Akiyama, M., Ando, M., ... & Akiyoshi, K. -
The thermotolerant yeast Kluyveromyces marxianus is a useful organism for structural and biochemical studies of autophagy.
Yamamoto, H., Shima, T., Yamaguchi, M., Mochizuki, Y., Hoshida, H., Kakuta, S., ... & Akada, R. -
Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria.
Ishii, E., Chiba, S., Hashimoto, N., Kojima, S., Homma, M., Ito, K., ... & Mori, H. -
Cotranslational protein folding inside the ribosome exit tunnel.
Nilsson, O. B., Hedman, R., Marino, J., Wickles, S., Bischoff, L., Johansson, M., ... & Beckmann, R. -
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Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system in Escherichia coli.
Awai T, Ichihashi N and Yomo T. -
Droplet‐Shooting and Size‐Filtration (DSSF) Method for Synthesis of Cell‐Sized Liposomes with Controlled Lipid Compositions.
Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., ... & Takinoue, M. -
Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device.
Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H., & Takinoue, M. -
In vitro directed evolution of alpha-hemolysin by liposome display.
Fujii S, Matsuura T and Yomo T. -
Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells.
van Nies, P., Canton, A. S., Nourian, Z., & Danelon, C. -
Simple Identification of Two Causes of Noise in an Aptazyme System by Monitoring Cell-Free Transcription
Ichihashi, N., Kobori, S., & Yomo, T. -
Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.
Hiroyuki Kogure, Yoshihiro Handa, Masahiro Nagata, Naoto Kanai, Peter Guntert, Kenji Kubota, and Nobukazu Nameki -
The PURE System for Protein Production.
Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda -
Protein synthesis yield increased 72 times in the cell-free PURE system
Jackson, K., Kanamori, T., Ueda, T., & Fan, Z. H. -
In vitro synthesis of the E. coli Sec translocon from DNA.
Matsubayashi, H., Kuruma, Y., & Ueda, T. -
Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.
Kazuta Y, Matsuura T, Ichihashi N and Yomo T. -
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PURE ribosome display and its application in antibody technology.
Kanamori T, Fujino Y and Ueda T. -
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Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system.
Kei Fujiwara, Tsutomu Katayama, and Shin-Ichiro M Nomura -
Translational halt during elongation caused by G-quadruplex formed by mRNA.
Tamaki Endoh, Yu Kawasaki, and Naoki Sugimoto -
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific non-standard amino acid incorporation.
Seok Hoon Hong, Ioanna Ntai, Adrian D Haimovich, Neil L Kelleher, Farren J Isaacs, and Michael C Jewett -
Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength.
Fabio Chizzolini, Michele Forlin, Dario Cecchi, and Sheref S Mansy -
In vitro evolution of a-hemolysin using a liposome display.
Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta, and Tetsuya Yomo -
Unbiased tracking of the progression of mRNA and protein synthesis in bulk and in liposome-confined reactions.
Pauline van Nies, Zohreh Nourian, Maurits Kok, Roeland van Wijk, Jonne Moeskops, Ilja Westerlaken, Jos M Poolman, Rienk Eelkema, Jan H van Esch, Yutetsu Kuruma, Takuya Ueda, and Christophe Danelon -
Implementation of cell-free biological networks at steady state.
Henrike Niederholtmeyer, Viktoria Stepanova, and Sebastian J Maerkl -
Fluorescent proteins and in vitro genetic organization for cell-free synthetic biology.
Roberta Lentini, Michele Forlin, Laura Martini, Cristina Del Bianco, Amy C Spencer, Domenica Torino, and Sheref S Mansy -
Nascent peptides that block protein synthesis in bacteria.
Christopher J Woolstenhulme, Shankar Parajuli, David W Healey, Diana P Valverde, E Nicholas Petersen, Agata L Starosta, Nicholas R Guydosh, W Evan Johnson, Daniel N Wilson, and Allen R Buskirk -
In vitro integration of ribosomal RNA synthesis; ribosome assembly; and translation.
Michael C Jewett, Brian R Fritz, Laura E Timmerman, and George M Church -
Real-time mRNA measurement during an in vitro transcription and translation reaction using binary probes.
Henrike Niederholtmeyer, Ling Xu, and Sebastian J Maerkl -
In vitro evolution of α-hemolysin using a liposome display.
Fujii S, Matsuura T, Sunami T, Kazuta Y and Yomo T. -
Stability of RNA quadruplex in open reading frame determines proteolysis of human estrogen receptor α.
Endoh, T., Kawasaki, Y., & Sugimoto, N. -
Suppression of Gene Expression by G‐Quadruplexes in Open Reading Frames Depends on G‐Quadruplex Stability.
Endoh, T., Kawasaki, Y., & Sugimoto, N. -
Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming.
Ki Baek Lee, Ho-Cheol Kim, Dong-Myung Kim, Taek Jin Kang, and Hiroaki Suga -
SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice.
Yasuhiko Nakamura, Masahito Ogura, Kasane Ogura, Daisuke Tanaka, and Nobuya Inagaki -
Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning.
Yasuhiro Fujino, Risako Fujita, Kouichi Wada, Kotomi Fujishige, Takashi Kanamori, Lindsey Hunt, Yoshihiro Shimizu, and Takuya Ueda -
Modification-free photocontrol of b-lactam conversion with spatiotemporal resolution.
Anna Venancio-Marques, Yan-Jun Liu, Antoine Diguet, Thomas di Maio, Arnaud Gautier, and Damien Baigl -
Nanoproteomics enabling personalized nanomedicine.
Claudio Nicolini, Nicola Bragazzi, and Eugenia Pechkova -
Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis.
Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Norikazu Ichihashi, Hiroaki Suzuki, and Tetsuya Yomo -
Analysis of p53 binding to DNA by fluorescence imaging microscopy.
Hwee Jing Ong, Jia Wei Siau, Jing Bo Zhang, Minghui Hong, Horst Flotow, and Farid Ghadessy -
ArfA recruits RF2 into stalled ribosomes.
Yoshihiro Shimizu -
Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli.
Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda, and Yoshitaka Nishiyama -
Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.
Colin J B Harvey, Joseph D Puglisi, Vijay S Pande, David E Cane, and Chaitan Khosla -
Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: Modeling of ligand-dependent and -independent expression.
Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, and Tetsuya Yomo -
Target specificity of an autoreactive pathogenic human gd-T cell receptor in myositis.
Jessica Bruder, Katherina Siewert, Birgit Obermeier, Joachim Malotka, Peter Scheinert, Josef Kellermann, Takuya Ueda, Reinhard Hohlfeld, and Klaus Dornmair -
Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry.
Koji Nishimura, Tomoaki Matsuura, Kazuya Nishimura, Takeshi Sunami, Hiroaki Suzuki, and Tetsuya Yomo -
Cell-free protein synthesis from a single copy of DNA in a glass microchamber.
Taiji Okano, Tomoaki Matsuura, Yasuaki Kazuta, Hiroaki Suzuki, and Tetsuya Yomo -
A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.
Cassandra Guarino, and Matthew P Delisa -
Experiment and mathematical modeling of gene expression dynamics in a cell-free system.
Tobias Stogbauer, Lukas Windhager, Ralf Zimmer, and Joachim O Radler -
Mutant p53 cooperates with ETS2 to promote etoposide resistance.
Phi M Do, Lakshman Varanasi, Songqing Fan, Chunyang Li, Iwona Kubacka, Virginia Newman, Krishna Chauhan, Silvano Rakeem Daniels, Maurizio Boccetta, Michael R Garrett, Runzhao Li, and Luis A Martinez -
Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation.
Verena Kriechbaumer, Pengwei Wang, Chris Hawes, and Ben M Abell -
Differential regulation of mesodermal gene expression by Drosophila cell type-specific Forkhead transcription factors.
Xianmin Zhu, Shaad M Ahmad, Anton Aboukhalil, Brian W Busser, Yongsok Kim, Terese R Tansey, Adrian Haimovich, Neal Jeffries, Martha L Bulyk, and Alan M Michelson -
In vitro selection of highly modified cyclic peptides that act as tight binding inhibitors. J. Am. Chem. Soc. 134 10469-10477.
Yollete V Guillen Schlippe, Matthew C T Hartman, Kristopher Josephson, and Jack W Szostak -
Traveling time of a translating ribosome along mRNA monitored directly on a quartz crystal microbalance.
Shuntaro Takahashi, Kentaro Tsuji, Takuya Ueda, and Yoshio Okahata -
The ancestral SgrS RNA discriminates horizontally acquired Salmonella mRNAs through a single G-U wobble pair.
Kai Papenfort, Dimitri Podkaminski, Jay C D Hinton, and Jorg Vogel -
Triggered gene expression in fed-vesicle microreactors with a multifunctional membrane.
Zohreh Nourian, Wouter Roelofsen, and Christophe Danelon -
Real-time assay for testing components of protein synthesis.
Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Yale E Goldman, and Barry S Cooperman -
Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells.
Kodai Machida, Mamiko Masutani, Tominari Kobayashi, Satoshi Mikami, Yuri Nishino, Atsuo Miyazawa, and Hiroaki Imataka -
Broad-specificity mRNA-rRNA complementarity in efficient protein translation.
Pamela A Barendt, Najaf A Shah, Gregory A Barendt, and Casim A Sarkar -
Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis.
Harris H Wang, Po-Yi Huang, George Xu, Wilhelm Haas, Adam Marblestone, Jun Li, Steven P Gygi, Anthony C Forster, Michael C Jewett, and George M Church -
A mixed double negative feedback loop between the sRNA MicF and the global regulator Lrp.
Erik Holmqvist, Cecilia Unoson, Johan Reimegard, and E Gerhart H Wagner -
Synchronized translation for detection of temporal stalling of ribosome during single-turnover translation.
Tamaki Endoh, Yu Kawasaki, and Naoki Sugimoto -
Clostridium difficile cell wall protein CwpV undergoes enzyme-independent intramolecular autoproteolysis.
Marcin Dembek, Catherine B Reynolds, and Neil F Fairweather -
A concept for selection of codon-suppressor tRNAs based on read-through ribosome display in an in vitro compartmentalized cell-free translation system.
Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando, and Yasuhiro Aoyama -
Characterization of the emergent properties of a synthetic quasi-cellular system.
Lorenzo Lazzerini-Ospri, Pasquale Stano, Pier Luigi Luisi, and Roberto Marangoni -
DNA display selection of peptide ligands for a full-length human g protein-coupled receptor on CHO-K1 cells.
Nobuhide Doi, Natsuko Yamakawa, Hideaki Matsumoto, Yasutsugu Yamamoto, Tetsuya Nagano, Nobutaka Matsumura, Kenichi Horisawa, and Hiroshi Yanagawa -
Synthesis of [11C]interleukin 8 using a cell-free translation system and L-[11C]methionine.
Ryuichi Harada, Shozo Furumoto, Takeo Yoshikawa, Yoichi Ishikawa, Katsuhiko Shibuya, Nobuyuki Okamura, Ren Iwata, and Kazuhiko Yanai -
Characterization of type II and III restriction-modification systems from Bacillus cereus strains ATCC 10987 and ATCC 14579.
Shuang-Yong Xu, Rebecca L Nugent, Julie Kasamkattil, Alexey Fomenkov, Yogesh Gupta, Aneel Aggarwal, Xiaolong Wang, Zhiru Li, Yu Zheng, and Richard Morgan -
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In vitro selection of unnatural cyclic peptide libraries via mRNA display.
Zhong Ma, and Matthew C T Hartman -
Peptide screening using PURE ribosome display.
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K Akbar, and Takuya Ueda -
Directed evolution of proteins through in vitro protein synthesis in liposomes.
Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, and Tetsuya Yomo -
In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR.
Takeshi Sumida, Hiroshi Yanagawa, and Nobuhide Doi -
Expression optimization and synthetic gene networks in cell-free systems.
David K Karig, Sukanya Iyer, Michael L Simpson, and Mitchel J Doktycz -
Global analysis of chaperone effects using a reconstituted cell-free translation system.
Niwa T, Kanamori T, Ueda T, Taguchi H. -
The ribosome modulates nascent protein folding.
Christian M Kaiser, Daniel H Goldman, John D Chodera, Ignacio Tinoco, and Carlos Bustamante -
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Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins.
Anna L Mallam, and Sophie E Jackson -
Zn(II) binding to E. coli 70S ribosomes.
Mart Patrick Hensley, David L Tierney, and Michael Wade Crowder -
Spontaneous crowding of ribosomes and proteins inside vesicles: a possible mechanism for the origin of cell metabolism. Chembiochem 12, 2325-2330.
Tereza Pereira de Souza, Frank Steiniger, Pasquale Stano, Alfred Fahr, and Pier Luigi Luisi -
Ribosome display selection of a murine IgG1 Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.
Sebastian Grimm, Feifan Yu, and Per-Ake Nygren -
In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.
Hayato Yanagida, Tomoaki Matsuura, Yasuaki Kazuta, and Tetsuya Yomo -
Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system.
John P Welsh, Jeanne Bonomo, and James R Swartz -
Polypeptide aptamer selection using a stabilized ribosome display.
Wei Wang, Shuta Hara, Mingzhe Liu, Toshiro Aigaki, Shigeru Shimizu, and Yoshihiro Ito -
Luminescence Resonance Energy Transfer in the Cytoplasm of Live Escherichia coli Cells.
Daniel Gonzalez, Nayanish Lokhande, Swaraj Vadde, Qi Zhao, Aaron Cassill, and Robert Renthal -
Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5°C.
Fukashi Kihara, Teruyuki Niimi, Okitsugu Yamashita, and Toshinobu Yaginuma -
Kinetic study of de novo chromophore maturation of fluorescent proteins.
Ryo Iizuka, Mai Yamagishi-Shirasaki, and Takashi Funatsu -
Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes.
Takuma Ohtsuka, Satoshi Neki, Tamotsu Kanai, Kazunari Akiyoshi, Shin-Ichiro M Nomura, and Takashi Ohtsuki -
Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays.
Kathy N Lam, Harm van Bakel, Atina G Cote, Anton van der Ven, and Timothy R Hughes -
Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants.
Federico De Masi, Christian A Grove, Anastasia Vedenko, Andreu Alibes, Stephen S Gisselbrecht, Luis Serrano, Martha L Bulyk, and Albertha J M Walhout -
tmRNA regulates synthesis of the ArfA ribosome rescue factor.
Fernando Garza-Sanchez, Ryan E Schaub, Brian D Janssen, and Christopher S Hayes -
Improvement of translation efficiency in an Escherichia coli cell-free protein system using cysteine.
Tomoteru Shingaki, and Noriyuki Nimura -
Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox.
K Rosner, M F Kasprzak, A C J Horenstein, H L Thurston, J Abrams, L Y Kerwin, D A Mehregan, and D R Mehregan -
Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome.
Zhan-Ping Zhou, Yoshihiro Shimizu, Hisashi Tadakuma, Hideki Taguchi, Koichi Ito, and Takuya Ueda -
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, and Koreaki Ito -
Identification of a chitin-induced small RNA that regulates translation of the tfoX gene; encoding a positive regulator of natural competence in Vibrio cholerae.
Shouji Yamamoto, Hidemasa Izumiya, Jiro Mitobe, Masatomo Morita, Eiji Arakawa, Makoto Ohnishi, and Haruo Watanabe -
Optimal codon choice can improve the efficiency and fidelity of N-methyl amino acid incorporation into peptides by in-vitro translation.
Alexander O Subtelny, Matthew C T Hartman, and Jack W Szostak -
YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes.
Yoshihiro Handa, Noriyuki Inaho, and Nobukazu Nameki -
Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center.
Haripriya Ramu, Nora Vazquez-Laslop, Dorota Klepacki, Qing Dai, Joseph Piccirilli, Ronald Micura, and Alexander S Mankin -
The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes.
Christakis Panayiotou, Nicola Solaroli, Yunjian Xu, Magnus Johansson, and Anna Karlsson -
Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP.
Vikram Narayan, Emmanuelle Pion, Vivien Landre, Petr Muller, and Kathryn L Ball -
Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries.
Tek N Lamichhane, N Dinuka Abeydeera, Anne-Cecile E Duc, Philip R Cunningham, and Christine S Chow -
Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae.
Marika Midon, Patrick Schafer, Alfred Pingoud, Mahua Ghosh, Andrea F Moon, Matthew J Cuneo, Robert E London, and Gregor Meiss -
Cortisol is involved in temperature-dependent sex determination in the Japanese flounder.
Toshiya Yamaguchi, Norifumi Yoshinaga, Takashi Yazawa, Koichiro Gen, and Takeshi Kitano -
Ribosome display with the PURE technology.
Takuya Ueda, Takashi Kanamori, and Hiroyuki Ohashi -
Production of multi-subunit complexes on liposome through an E. coli cell-free expression system.
Yutetsu Kuruma, Toshiharu Suzuki, and Takuya Ueda -
PURE technology.
Yoshihiro Shimizu, and Takuya Ueda -
Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation.
Yuki Moritani, Shin-ichiro M Nomura, Ikuo Morita, and Kazunari Akiyoshi -
Versatility of trigger factor interactions with ribosome-nascent chain complexes.
Sathish Kumar Lakshmipathy, Rashmi Gupta, Stefan Pinkert, Stephanie Anne Etchells, and F Ulrich Hartl -
In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme.
Haruichi Asahara, and Shaorong Chong -
The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex.
Gandhi Theerthagiri, Nathalie Eisenhardt, Heinz Schwarz, and Wolfram Antonin -
Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI.
Betty W Shen, Daniel F Heiter, Siu-Hong Chan, Hua Wang, Shuang-Yong Xu, Richard D Morgan, Geoffrey G Wilson, and Barry L Stoddard -
Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis.
Erik Holmqvist, Johan Reimegard, Maaike Sterk, Nina Grantcharova, Ute Romling, and Eduard Gerhart Heinrich Wagner -
Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions.
Takeshi Sunami, Kazufumi Hosoda, Hiroaki Suzuki, Tomoaki Matsuura, and Tetsuya Yomo -
Comparing the functional properties of the Hsp70 chaperones; DnaK and BiP.
Jeanne Bonomo, John P Welsh, Karthish Manthiram, and James R Swartz -
A novel complete reconstitution system for membrane integration of the simplest membrane protein.
Ken-Ichi Nishiyama, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda, and Hajime Tokuda -
The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus.
Tomoko Noto, Henriette M Kurth, Kensuke Kataoka, Lucia Aronica, Leroi V DeSouza, K W Michael Siu, Ronald E Pearlman, Martin A Gorovsky, and Kazufumi Mochizuki -
mRNA display selection of a high-affinity; Bcl-XL-specific binding peptide.
Nobutaka Matsumura, Toru Tsuji, Takeshi Sumida, Masahito Kokubo, Michiko Onimaru, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato, and Hiroshi Yanagawa -
Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.
Osada E, Shimizu Y, Akbar BK, Kanamori T, Ueda T. -
Genetic identification of nascent peptides that induce ribosome stalling.
Douglas R Tanner, Daniel A Cariello, Christopher J Woolstenhulme, Mark A Broadbent, and Allen R Buskirk -
Bicistronic DNA display for in vitro selection of Fab fragments.
Takeshi Sumida, Nobuhide Doi, and Hiroshi Yanagawa -
Profiling of gene-dependent translational progress in cell-free protein synthesis by real-space imaging.
Eriko Mikamo-Satoh, Akihiko Takagi, Hiroyuki Tanaka, Takuya Matsumoto, Tsutomu Nishihara, and Tomoji Kawai -
Roles of Tom70 in import of presequence-containing mitochondrial proteins.
Hayashi Yamamoto, Kenji Fukui, Hisashi Takahashi, Shingo Kitamura, Takuya Shiota, Kayoko Terao, Mayumi Uchida, Masatoshi Esaki, Shuh-ichi Nishikawa, Tohru Yoshihisa, Koji Yamano, and Toshiya Endo -
Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation.
Nora Gockler, Guillermo Jofre, Chrisovalantis Papadopoulos, Ulf Soppa, Francisco J Tejedor, and Walter Becker -
Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]
Ikuo Uchida, Ryoko Ishihara, Kiyoshi Tanaka, Eiji Hata, Sou-ichi Makino, Toru Kanno, Shinichi Hatama, Masato Kishima, Masato Akiba, Atsushi Watanabe, and Takayuki Kubota -
A new member of the Escherichia coli fad regulon: transcriptional regulation of fadM (ybaW).
Youjun Feng, and John E Cronan -
Identification of two active functional domains of human adenylate kinase 5.
Nicola Solaroli, Christakis Panayiotou, Magnus Johansson, and Anna Karlsson -
Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation.
Verena Pfeiffer, Kai Papenfort, Sacha Lucchini, Jay C D Hinton, and Jorg Vogel -
Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder.
Andre Estevez-Torres, Cecile Crozatier, Antoine Diguet, Tomoaki Hara, Hirohide Saito, Kenichi Yoshikawa, and Damien Baigl -
Interleukin-33 is biologically active independently of caspase-1 cleavage.
Dominique Talabot-Ayer, Celine Lamacchia, Cem Gabay, and Gaby Palmer -
Real-time monitoring of cell-free translation on a quartz-crystal microbalance.
Shuntaro Takahashi, Masaaki Iida, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda,and Yoshio Okahata -
Analysis of nonstop mRNA translation in the absence of tmRNA in Escherichia coli.
Kazushige Kuroha, Nobuko Horiguchi, Hiroji Aiba, and Toshifumi Inada -
Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.
Eriko Osada, Yoshihiro Shimizu, Bintang K Akbar, Takashi Kanamori, and Takuya Ueda -
Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins.
Tatsuya Niwa, Bei-Wen Ying, Katsuyo Saito, WenZhen Jin, Shoji Takada, Takuya Ueda, and Hideki Taguchi -
A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells.
Yutetsu Kuruma, Pasquale Stano, Takuya Ueda, and Pier Luigi Luisi -
Trigger factor from the psychrophilic bacterium Psychrobacter frigidicola is a monomeric chaperone.
Sylvain Robin, Denisio M Togashi, Alan G Ryder, and J Gerard Wall -
Quantifying epistatic interactions among the components constituting the protein translation system.
Tomoaki Matsuura, Yasuaki Kazuta, Takuyo Aita, Jiro Adachi, and Tetsuya Yomo -
Using shotgun sequence data to find active restriction enzyme genes.
Yu Zheng, Janos Posfai, Richard D Morgan, Tamas Vincze, and Richard J Roberts -
Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction.
Kazufumi Hosoda, Takeshi Sunami, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo -
Cell-free synthesis of the torque-generating membrane proteins; PomA and PomB; of the Na+-driven flagellar motor in Vibrio alginolyticus.
Hiroyuki Terashima, Rei Abe-Yoshizumi, Seiji Kojima, and Michio Homma -
Comprehensive analysis of the effects of Escherichia coli ORFs on protein translation reaction.
Yasuaki Kazuta, Jiro Adachi, Tomoaki Matsuura, Naoaki Ono, Hirotada Mori, and Tetsuya Yomo -
RNA; but not protein partners; is directly responsible for translational silencing by a bacterial Hfq-binding small RNA.
Kimika Maki, Kanako Uno, Teppei Morita, and Hiroji Aiba -
Initiating translation with D-amino acids.
Yuki Goto, Hiroshi Murakami, and Hiroaki Suga -
Single-molecule imaging of full protein synthesis by immobilized ribosomes.
Sotaro Uemura, Ryo Iizuka, Taro Ueno, Yoshihiro Shimizu, Hideki Taguchi, Takuya Ueda, Joseph D Puglisi, and Takashi Funatsu -
Single-molecule imaging of full protein synthesis by immobilized ribosomes.
Sotaro Uemura, Ryo Iizuka, Taro Ueno, Yoshihiro Shimizu, Hideki Taguchi, Takuya Ueda, Joseph D Puglisi, and Takashi Funatsu -
Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions.
Yusuke Sako, Jumpei Morimoto, Hiroshi Murakami, and Hiroaki Suga -
A general and rapid cell-free approach for the interrogation of protein-protein; protein-DNA; and protein-RNA interactions and their antagonists utilizing split-protein reporters.
Jason R Porter, Cliff I Stains, Benjamin W Jester, and Indraneel Ghosh -
Ribosomal synthesis of N-methyl peptides.
Alexander O Subtelny, Matthew C T Hartman, and Jack W Szostak -
Molecular mechanism of drug-dependent ribosome stalling.
Nora Vazquez-Laslop, Celine Thum, and Alexander S Mankin -
Ribosomal synthesis of peptidase-resistant peptides closed by a nonreducible inter-side-chain bond. ACS Chem. Biol. , 241-249.
Yusuke Sako, Yuki Goto, Hiroshi Murakami, and Hiroaki Suga -
Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
Johannes H Urban, and Jorg Vogel -
UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro.
Yoko Ozaki, Toshiharu Suzuki, Yutetsu Kuruma, Takuya Ueda, and Masasuke Yoshida -
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin.
Akihiko Sakamoto, Mai Yamagishi, Takafumi Watanabe, Youichi Aizawa, Takashi Kato, and Takashi Funatsu -
Reprogramming the translation initiation for the synthesis of physiologically stable cyclic peptides.
Yuki Goto, Atsushi Ohta, Yusuke Sako, Yusuke Yamagishi, Hiroshi Murakami, and Hiroaki Suga -
Messenger RNA-programmed incorporation of multiple N-methyl-amino acids into linear and cyclic peptides.
Takashi Kawakami, Hiroshi Murakami, and Hiroaki Suga -
The BsaHI restriction-modification system: cloning; sequencing and analysis of conserved motifs.
Robert K Neely, and Richard J Roberts -
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based.
Jason R Hillebrecht, and Shaorong Chong -
Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue.
Hayato Yanagida, Tomoaki Matsuura, and Tetsuya Yomo -
Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface
Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K. -
A monoclonal Fab derived from a human nonimmune phage library reveals a new epitope on gp41 and neutralizes diverse human immunodeficiency virus type 1 strains
Gustchina E, Louis JM, Lam SN, Bewley CA, Clore GM. -
Selection of vimentin-specific antibodies from the HuCALR phage display library by subtractive panning on formalin-fixed,paraffin-embedded tissue
Jarutat T, Nickels C, Frisch C, Stellmacher F, Hofig KP, Knappik A, Merz H. -
Synthesis of polyester by means of genetic code reprogramming.
Atsushi Ohta, Hiroshi Murakami, Eri Higashimura, and Hiroaki Suga -
Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system.
Yoshio Doi, Takashi Ohtsuki, Yoshihiro Shimizu, Takuya Ueda, and Masahiko Sisido -
Protein synthesis in liposomes with a minimal set of enzymes.
Giovanni Murtas, Yutetsu Kuruma, Paolo Bianchini, Alberto Diaspro, and Pier Luigi Luisi -
A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites.
Cynthia M Sharma, Fabien Darfeuille, Titia H Plantinga, and Jorg Vogel -
Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II.
Kouji Kojima, Masaru Oshita, Yohei Nanjo, Koji Kasai, Yuzuru Tozawa, Hidenori Hayashi, and Yoshitaka Nishiyama -
Unexpected preference of the E. coli translation system for the ester bond during incorporation of backbone-elongated substrates.
Shinsuke Sando, Kenji Abe, Nobuhiko Sato, Toshihiro Shibata, Keigo Mizusawa, and Yasuhiro Aoyama -
Identification of nascent chain interaction sites on trigger factor.
Sathish K Lakshmipathy, Sladjana Tomic, Christian M Kaiser, Hung-Chun Chang, Pierre Genevaux, Costa Georgopoulos, Jose M Barral, Arthur E Johnson, F Ulrich Hartl, and Stephanie A Etchells -
Nascent chain; mRNA; and ribosome complexes generated by a pure translation system.
Tomoaki Matsuura, Hayato Yanagida, Junya Ushioda, Itaru Urabe, and Tetsuya Yomo -
Efficient protein selection based on ribosome display system with purified components.
Hiroyuki Ohashi, Yoshihiro Shimizu, Bei-Wen Ying, and Takuya Ueda -
An mRNA-protein fusion at N-terminus for evolutionary protein engineering. Int.
Shingo Ueno, Hidenao Arai, Miho Suzuki, and Yuzuru Husimi -
Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution.
Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando, and Yasuhiro Aoyama -
Structural and functional definition of the specificity of a novel caspase-3 inhibitor
Atsushi Yoshimori, Junichi Sakai, Satoshi Sunaga, Takanobu Kobayashi, Satoshi Takahashi, Naoyuki Okita, Ryoko Takasawa, and Sei-ichi Tanuma -
High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system.
Yuko Kawahashi, Nobuhide Doi, Yo Oishi, Chizuru Tsuda, Hideaki Takashima, Tomoya Baba, Hirotada Mori, Takashi Ito, and Hiroshi Yanagawa -
Ribosomal protein S1 influences trans-translation in vitro and in vivo.
Matthieu Saguy, Reynald Gillet, Patricia Skorski, Sylvie Hermann-Le Denmat, and Brice Felden -
Selection of restriction endonucleases using artificial cells.
Yu Zheng, and Richard J Roberts -
Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction.
Kolly R, Thiel MA, Herrmann T, Pluckthun A. -
Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.
Kiyoko Setoguchi, Hidenori Otera, and Katsuyoshi Mihara -
Femtoliter compartment in liposomes for in vitro selection of proteins.
Takeshi Sunami, Kanetomo Sato, Tomoaki Matsuura, Koji Tsukada, Itaru Urabe, and Tetsuya Yomo -
Co-translational binding of GroEL to nascent polypeptides is followed by post-translational encapsulation by GroES to mediate protein folding.
Bei-Wen Ying, Hideki Taguchi, and Takuya Uedan -
Regulation of mitochondrial morphology through proteolytic cleavage of OPA1.
Naotada Ishihara, Yuu Fujita, Toshihiko Oka, and Katsuyoshi Miharan -
Affinity maturation of phage display antibody populations using ribosome display.
Maria Groves, Steven Lane, Julie Douthwaite, David Lowne, D Gareth Rees, Bryan Edwards, and Ronald H Jackson -
Highly efficient ribosome display selection by use of purified components for in vitro translation.
Denis Villemagne, Ronald Jackson, and Julie A Douthwaite -
Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli.
Tatsuya Yamamoto, Shunsuke Izumi, and Kunihiko Gekko -
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Ribosomal synthesis of dehydroalanine-containing peptides.
Florian P Seebeck, and Jack W Szostak -
Cytosolic chaperonin protects folding intermediates of Gbeta from aggregation by recognizing hydrophobic beta-strands. Proc. Natl.
Susumu Kubota, Hiroshi Kubota, and Kazuhiro Nagata -
Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall.
Hiroki Muto, Hitoshi Nakatogawa, and Koreaki Ito -
A highly flexible tRNA acylation method for non-natural polypeptide synthesis.
Hiroshi Murakami, Atsushi Ohta, Hiroshi Ashigai, and Hiroaki Suga -
Spermidine inhibits transient and stable ribosome subunit dissociation.
So Umekage, and Takuya Ueda -
Enhancement of protein synthesis by an inorganic polyphosphate in an E. coli cell-free system.
Hiromichi Itoh, Yumi Kawazoe, and Toshikazu Shiba -
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Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains.
Sladjana Tomic, Arthur E Johnson, F Ulrich Hartl, and Stephanie A Etchells -
Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation.
Marc Hallier, Jessie Desreac, and Brice Felden -
Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions
Vanhoutteghem, A., Djian, P. -
Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library
Jarutat, T., Frisch, C., Nickels, C., Merz, H., Knappik, A. -
Antibodies for proteomic research : Comparison of traditional immunization with recombinant antibody technology
Ohara, R., Knappik, A., Shimada, K., Frisch, C., Ylera, F., Koga, H.（かずさＤＮＡ研究所 古閑先生） -
Establishment of specific antibodies that recognize C106-oxidized DJ-1
Ooe, H., Iguchi-Ariga, S.M., Ariga, H.（北海道大学 薬学系研究院 有賀先生） -
Ribosomal synthesis of unnatural peptides.
Kristopher Josephson, Matthew C T Hartman, and Jack W Szostak -
Protein synthesis by pure translation systems
Yoshihiro Shimizu, Takashi Kanamori, and Takuya Ueda -
A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level.
Shinsuke Sando, Keiichiro Kanatani, Nobuhiko Sato, Hironori Matsumoto, Takahiro Hohsaka, and Yasuhiro Aoyama -
Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.
Keiko Fukushima, Yukio Ikehara, and Katsuko Yamashita -
Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation.
Masato Yano, Hirotaka J Okano, and Hideyuki Okano -
Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides.
Bei-Wen Ying, Hideki Taguchi, Mayumi Kondo, and Takuya Ueda -
Novel processing and localization of catA; ccdA associated thiol-disulfide oxidoreductase; in protein hyper-producing bacterium Brevibacillus choshinensis.
Ryoichi Tanaka, Makoto Mizukami, and Masao Tokunaga -
Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins.
Yutetsu Kuruma, Ken-Ichi Nishiyama, Yoshihiro Shimizu, Matthias Muller, and Takuya Ueda -
Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system.
Bei-Wen Ying, Hideki Taguchi, Hiroshi Ueda, and Takuya Ueda -
Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import.
Takeyoshi Asai, Takashi Takahashi, Masatoshi Esaki, Shuh-Ichi Nishikawa, Kenzo Ohtsuka, Masato Nakai, and Toshiya Endo -
Bulge- and basal layer-specific expression of fibroblast growth factor-13 (FHF-2) in mouse skin.
Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko Oki, and Toru Imamura -
Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.
Tsuyoshi Udagawa, Yoshihiro Shimizu, and Takuya Ueda -
A novel screening system for self-mRNA targeting proteins.
Bei-Wen Ying, Tsutomu Suzuki, Yoshihiro Shimizu, and Takuya Ueda -
Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3
Rauchenberger, R., Borges, E., Thomassen-Wolf, E., Rom, E., Adar, R., Yaniv, Y., Malka, M., Chumakov, I., Kotzer, S., Resnitzky, D., Knappik, A., Reiffert, S., Prassler, J., Jury, K. , Waldherr, D., Bauer, S., Kretzschmar, T., Yayon, A., Rothe, C. -
Cell-free translation reconstituted with purified components.
Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, and Takuya Ueda
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